The gating cycle of a K+ channel at atomic resolution
- Texas Tech University Health Sciences Center, United States
- The University of Chicago, United States
Abstract
C-type inactivation in potassium channels helps fine-tune long term channel activity through conformational changes at the selectivity filter. Here, through the use of cross-linked constitutively open constructs, we determined the structures of KcsA's mutants that stabilize the selectivity filter in its conductive (E71A, at 2.25 Å) and deep C-type inactivated (Y82A at 2.4 Å) conformations. These structural snapshots represent KcsA's transient open-conductive (O/O) and the stable open deep C-type inactivated states (O/I), respectively. The present structures provide an unprecedented view of the selectivity filter backbone in its collapsed deep C-type inactivated conformation, highlighting the close interactions with structural waters and the local allosteric interactions that couple activation and inactivation gating. Together with the structures associated with the closed-inactivated state (C/I) and in the well-known closed conductive state (C/O), this work recapitulates, at atomic resolution, the key conformational changes of a potassium channel pore domain as it progresses along its gating cycle.
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Author details
Funding
National Institute of General Medical Sciences (U54 GM087519)
- Eduardo Perozo
Welch Foundation (BI-1757)
- Luis G Cuello
National Institute of General Medical Sciences (1RO1GM097159-01A1)
- Luis G Cuello
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Cuello et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The gating cycle of a K+ channel at atomic resolution
eLife 6:e28032.
https://doi.org/10.7554/eLife.28032
