TY - JOUR TI - High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast AU - Yin, Yi AU - Dominska, Margaret AU - Yim, Eunice AU - Petes, Thomas D A2 - de Massy, Bernard VL - 6 PY - 2017 DA - 2017/07/17 SP - e28069 C1 - eLife 2017;6:e28069 DO - 10.7554/eLife.28069 UR - https://doi.org/10.7554/eLife.28069 AB - In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination. KW - mitotic recombination KW - heteroduplex DNA KW - genome-wide mapping KW - mismatch repair KW - gene conversion KW - genome rearrangements JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -