TY - JOUR TI - A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis AU - Altemose, Nicolas AU - Noor, Nudrat AU - Bitoun, Emmanuelle AU - Tumian, Afidalina AU - Imbeault, Michael AU - Chapman, J Ross AU - Aricescu, A Radu AU - Myers, Simon R A2 - Przeworski, Molly VL - 6 PY - 2017 DA - 2017/10/26 SP - e28383 C1 - eLife 2017;6:e28383 DO - 10.7554/eLife.28383 UR - https://doi.org/10.7554/eLife.28383 AB - PRDM9 binding localizes almost all meiotic recombination sites in humans and mice. However, most PRDM9-bound loci do not become recombination hotspots. To explore factors that affect binding and subsequent recombination outcomes, we mapped human PRDM9 binding sites in a transfected human cell line and measured PRDM9-induced histone modifications. These data reveal varied DNA-binding modalities of PRDM9. We also find that human PRDM9 frequently binds promoters, despite their low recombination rates, and it can activate expression of a small number of genes including CTCFL and VCX. Furthermore, we identify specific sequence motifs that predict consistent, localized meiotic recombination suppression around a subset of PRDM9 binding sites. These motifs strongly associate with KRAB-ZNF protein binding, TRIM28 recruitment, and specific histone modifications. Finally, we demonstrate that, in addition to binding DNA, PRDM9's zinc fingers also mediate its multimerization, and we show that a pair of highly diverged alleles preferentially form homo-multimers. KW - meiosis KW - recombination KW - zinc finger protein KW - transposable elements KW - PRDM9 KW - KRAB JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -