TY - JOUR TI - Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish AU - Fukui, Hajime AU - Miyazaki, Takahiro AU - Chow, Renee Wei-Yan AU - Ishikawa, Hiroyuki AU - Nakajima, Hiroyuki AU - Vermot, Julien AU - Mochizuki, Naoki A2 - Yelon, Deborah VL - 7 PY - 2018 DA - 2018/05/29 SP - e29106 C1 - eLife 2018;7:e29106 DO - 10.7554/eLife.29106 UR - https://doi.org/10.7554/eLife.29106 AB - The differentiation of the lateral plate mesoderm cells into heart field cells constitutes a critical step in the development of cardiac tissue and the genesis of functional cardiomyocytes. Hippo signaling controls cardiomyocyte proliferation, but the role of Hippo signaling during early cardiogenesis remains unclear. Here, we show that Hippo signaling regulates atrial cell number by specifying the developmental potential of cells within the anterior lateral plate mesoderm (ALPM), which are incorporated into the venous pole of the heart tube and ultimately into the atrium of the heart. We demonstrate that Hippo signaling acts through large tumor suppressor kinase 1/2 to modulate BMP signaling and the expression of hand2, a key transcription factor that is involved in the differentiation of atrial cardiomyocytes. Collectively, these results demonstrate that Hippo signaling defines venous pole cardiomyocyte number by modulating both the number and the identity of the ALPM cells that will populate the atrium of the heart. KW - cardiogenesis KW - Hippo KW - heart field KW - islet-1 KW - Lats kinase KW - Yap JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -