TY - JOUR TI - aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts AU - Hannaford, Matthew Robert AU - Ramat, Anne AU - Loyer, Nicolas AU - Januschke, Jens A2 - Yamashita, Yukiko M VL - 7 PY - 2018 DA - 2018/01/24 SP - e29939 C1 - eLife 2018;7:e29939 DO - 10.7554/eLife.29939 UR - https://doi.org/10.7554/eLife.29939 AB - Cell fate assignment in the nervous system of vertebrates and invertebrates often hinges on the unequal distribution of molecules during progenitor cell division. We address asymmetric fate determinant localization in the developing Drosophila nervous system, specifically the control of the polarized distribution of the cell fate adapter protein Miranda. We reveal a step-wise polarization of Miranda in larval neuroblasts and find that Miranda’s dynamics and cortical association are differently regulated between interphase and mitosis. In interphase, Miranda binds to the plasma membrane. Then, before nuclear envelope breakdown, Miranda is phosphorylated by aPKC and displaced into the cytoplasm. This clearance is necessary for the subsequent establishment of asymmetric Miranda localization. After nuclear envelope breakdown, actomyosin activity is required to maintain Miranda asymmetry. Therefore, phosphorylation by aPKC and differential binding to the actomyosin network are required at distinct phases of the cell cycle to polarize fate determinant localization in neuroblasts. KW - polarity KW - asymmetric cell division KW - neuroblasts KW - Drosophila JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -