TY - JOUR TI - Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle AU - Dasgupta, Agnik AU - Merkel, Matthias AU - Clark, Madeline J AU - Jacob, Andrew E AU - Dawson, Jonathan Edward AU - Manning, M Lisa AU - Amack, Jeffrey D A2 - Bronner, Marianne VL - 7 PY - 2018 DA - 2018/01/29 SP - e30963 C1 - eLife 2018;7:e30963 DO - 10.7554/eLife.30963 UR - https://doi.org/10.7554/eLife.30963 AB - How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer’s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces. KW - Kupffer's Vesicle KW - epithelial cell shape changes KW - cell volume regulation KW - mechanical influence on cell shapes JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -