TY - JOUR TI - Coupling chemosensory array formation and localization AU - Alvarado, Alejandra AU - Kjær, Andreas AU - Yang, Wen AU - Mann, Petra AU - Briegel, Ariane AU - Waldor, Matthew K AU - Ringgaard, Simon A2 - Mignot, Tâm VL - 6 PY - 2017 DA - 2017/10/23 SP - e31058 C1 - eLife 2017;6:e31058 DO - 10.7554/eLife.31058 UR - https://doi.org/10.7554/eLife.31058 AB - Chemotaxis proteins organize into large, highly ordered, chemotactic signaling arrays, which in Vibrio species are found at the cell pole. Proper localization of signaling arrays is mediated by ParP, which tethers arrays to a cell pole anchor, ParC. Here we show that ParP’s C-terminus integrates into the core-unit of signaling arrays through interactions with MCP-proteins and CheA. Its intercalation within core-units stimulates array formation, whereas its N-terminal interaction domain enables polar recruitment of arrays and facilitates its own polar localization. Linkage of these domains within ParP couples array formation and localization and results in controlled array positioning at the cell pole. Notably, ParP’s integration into arrays modifies its own and ParC’s subcellular localization dynamics, promoting their polar retention. ParP serves as a critical nexus that regulates the localization dynamics of its network constituents and drives the localized assembly and stability of the chemotactic machinery, resulting in proper cell pole development. KW - Vibrio cholerae KW - Chemotaxis KW - ParP KW - array formation KW - array localization KW - ParC JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -