TY - JOUR TI - Neurogliaform cortical interneurons derive from cells in the preoptic area AU - Niquille, Mathieu AU - Limoni, Greta AU - Markopoulos, Foivos AU - Cadilhac, Christelle AU - Prados, Julien AU - Holtmaat, Anthony AU - Dayer, Alexandre A2 - Gleeson, Joseph G VL - 7 PY - 2018 DA - 2018/03/20 SP - e32017 C1 - eLife 2018;7:e32017 DO - 10.7554/eLife.32017 UR - https://doi.org/10.7554/eLife.32017 AB - Delineating the basic cellular components of cortical inhibitory circuits remains a fundamental issue in order to understand their specific contributions to microcircuit function. It is still unclear how current classifications of cortical interneuron subtypes relate to biological processes such as their developmental specification. Here we identified the developmental trajectory of neurogliaform cells (NGCs), the main effectors of a powerful inhibitory motif recruited by long-range connections. Using in vivo genetic lineage-tracing in mice, we report that NGCs originate from a specific pool of 5-HT3AR-expressing Hmx3+ cells located in the preoptic area (POA). Hmx3-derived 5-HT3AR+ cortical interneurons (INs) expressed the transcription factors PROX1, NR2F2, the marker reelin but not VIP and exhibited the molecular, morphological and electrophysiological profile of NGCs. Overall, these results indicate that NGCs are a distinct class of INs with a unique developmental trajectory and open the possibility to study their specific functional contribution to cortical inhibitory microcircuit motifs. KW - Cortex KW - development KW - interneurons JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -