TY - JOUR TI - A sleep state in Drosophila larvae required for neural stem cell proliferation AU - Szuperak, Milan AU - Churgin, Matthew A AU - Borja, Austin J AU - Raizen, David M AU - Fang-Yen, Christopher AU - Kayser, Matthew S A2 - Ramaswami, Mani VL - 7 PY - 2018 DA - 2018/02/09 SP - e33220 C1 - eLife 2018;7:e33220 DO - 10.7554/eLife.33220 UR - https://doi.org/10.7554/eLife.33220 AB - Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation. KW - Drosophila KW - sleep KW - larva KW - neurogenesis KW - development JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -