TY - JOUR TI - Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses AU - Schanzenbächer, Christoph T AU - Langer, Julian D AU - Schuman, Erin M A2 - Goda, Yukiko VL - 7 PY - 2018 DA - 2018/02/15 SP - e33322 C1 - eLife 2018;7:e33322 DO - 10.7554/eLife.33322 UR - https://doi.org/10.7554/eLife.33322 AB - In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells with either TTX or bicucculline for 2 hr to induce the process of up- or down-scaling, respectively. During the activity manipulation we metabolically labeled newly synthesized proteins using BONCAT. We identified 168 newly synthesized proteins that exhibited significant changes in expression. To obtain a temporal trajectory of the response, we compared the proteins synthesized within 2 hr or 24 hr of the activity manipulation. Surprisingly, there was little overlap in the significantly regulated newly synthesized proteins identified in the early- and integrated late response datasets. There was, however, overlap in the functional categories that are modulated early and late. These data indicate that within protein function groups, different proteomic choices can be made to effect early and late homeostatic responses that detect the duration and polarity of the activity manipulation. KW - proteomics KW - homeostasis KW - synaptic plasticity KW - synaptic scaling JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -