TY - JOUR TI - A conserved function for pericentromeric satellite DNA AU - Jagannathan, Madhav AU - Cummings, Ryan AU - Yamashita, Yukiko M A2 - VijayRaghavan, K VL - 7 PY - 2018 DA - 2018/03/26 SP - e34122 C1 - eLife 2018;7:e34122 DO - 10.7554/eLife.34122 UR - https://doi.org/10.7554/eLife.34122 AB - A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi-AT-hook satellite DNA-binding proteins, Drosophila melanogaster D1 and mouse HMGA1, play an evolutionarily conserved role in bundling pericentromeric satellite DNA from heterologous chromosomes into ‘chromocenters’, a cytological association of pericentromeric heterochromatin. Defective chromocenter formation leads to micronuclei formation due to budding from the interphase nucleus, DNA damage and cell death. We propose that chromocenter and satellite DNA serve a fundamental role in encapsulating the full complement of the genome within a single nucleus, the universal characteristic of eukaryotic cells. KW - Satellite DNA KW - chromocenter KW - micronuclei KW - pericentromeric heterochromatin JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -