The functional organization of descending sensory-motor pathways in Drosophila

  1. Shigehiro Namiki
  2. Michael H Dickinson
  3. Allan M Wong
  4. Wyatt Korff
  5. Gwyneth M Card  Is a corresponding author
  1. Janelia Research Campus, Howard Hughes Medical Institute, United States
  2. California Institute of Technology, United States
15 figures, 1 table and 7 additional files

Figures

Strategy for identifying descending neurons.

(A, B) The fly central nervous system includes the brain, located in the fly’s head, and a ventral nerve cord (VNC), located in the fly’s thoracic cavity. These are connected by a population of …

https://doi.org/10.7554/eLife.34272.002
Figure 2 with 13 supplements
Reconstruction of identified descending neurons.

Morphology of descending neurons identified in the present study. Neurons (black) and neuropil regions of the brain and VNC are shown (transparent). A total of 98 different cell types are shown. …

https://doi.org/10.7554/eLife.34272.003
Figure 2—figure supplement 1
Morphology of DNs, group a.

Morphology of identified DNs, group a, whose somata are clustered dorsally on the anterior surface of the brain. For Figure 2—figure supplements 113, images are masks for single DNs made from …

https://doi.org/10.7554/eLife.34272.004
Figure 2—figure supplement 2
Morphology of DNs, group b.

Morphology of identified DNs, group b, whose somata are clustered ventrally on the anterior surface of the brain.

https://doi.org/10.7554/eLife.34272.005
Figure 2—figure supplement 3
Morphology of DNs, group c.

Morphology of identified DNs, group c, whose somata are located in the pars intercerebralis.

https://doi.org/10.7554/eLife.34272.006
Figure 2—figure supplement 4
Morphology of DNs, group d.

Morphology of identified DNs, group d, whose somata are located in an outside cluster on the anterior surface of the brain.

https://doi.org/10.7554/eLife.34272.007
Figure 2—figure supplement 5
Morphology of DNs, group p (DNp01-11).

Morphology of identified DNs, group p, whose somata are located on the posterior surface of the brain (1/4).

https://doi.org/10.7554/eLife.34272.008
Figure 2—figure supplement 6
Morphology of DNs, group p (DNp12-22).

Morphology of identified DNs, group p, whose somata are located on the posterior surface of the brain (2/4).

https://doi.org/10.7554/eLife.34272.009
Figure 2—figure supplement 7
Morphology of DNs, group p (DNp23-29).

Morphology of identified DNs, group p, whose somata are located on the posterior surface of the brain (3/4).

https://doi.org/10.7554/eLife.34272.010
Figure 2—figure supplement 8
Morphology of DNs, group p (DNp30-35).

Morphology of identified DNs, group p, whose somata are located on the posterior surface of the brain (4/4).

https://doi.org/10.7554/eLife.34272.011
Figure 2—figure supplement 9
Morphology of DNs, group g (DNg01-12)

Morphology of identified DNs, group g, whose somata are located on the gnathal ganglion (1/4).

https://doi.org/10.7554/eLife.34272.012
Figure 2—figure supplement 10
Morphology of DNs, group g (DNg13-24).

Morphology of identified DNs, group g, whose somata are located on the gnathal ganglion (2/4).

https://doi.org/10.7554/eLife.34272.013
Figure 2—figure supplement 11
Morphology of DNs, group g (DNg25-34).

Morphology of identified DNs, group g, whose somata are located on the gnathal ganglion (3/4).

https://doi.org/10.7554/eLife.34272.014
Figure 2—figure supplement 12
Morphology of DNs, group g (DNg35-41).

Morphology of identified DNs, group g, whose somata are located on the gnathal ganglion (4/4).

https://doi.org/10.7554/eLife.34272.015
Figure 2—figure supplement 13
Morphology of DNs, group x.

Morphology of identified DN, group x, whose somata is located outside the brain.

https://doi.org/10.7554/eLife.34272.016
Figure 3 with 1 supplement
Unique and population descending neurons.

(A) Three example morphologies of DNs that are uniquely identifiable (DNa05, DNp25 and DNp06). Maximum intensity projection images for brain (top) and VNC (bottom) are shown. (B) Three examples of …

https://doi.org/10.7554/eLife.34272.017
Figure 3—figure supplement 1
Further examples of population DNs.

(A) An example of the morphology of the DNg07 population. (B) Confocal image of brain innervation for the sample with six pairs of DNg07 type neurons. (C) Confocal image of brain innervation for the …

https://doi.org/10.7554/eLife.34272.018
Figure 4 with 2 supplements
Anatomical compartments of the brain and VNC in Drosophila.

(A–D) Identified brain neuropils labeled with different colors superimposed on an aligned confocal image. Depth from the anterior surface is indicated in top-right of each image. The data is from …

https://doi.org/10.7554/eLife.34272.019
Figure 4—figure supplement 1
Morphology of interneurons innervating the wing neuropil.

(A–E) Isolated morphologies of wing muscle motor neurons for (A) steering muscle b1 (mnb1), (B) steering muscle b2 (putative), (C) a dorsoventral power muscle (DVM), as well as two wing neuropil …

https://doi.org/10.7554/eLife.34272.020
Figure 4—figure supplement 2
Morphology of interneurons innervating the lower tectulum.

(A–E) Isolated morphologies for motorneurons and interneurons innervating the lower tectulum, including two neurons postsynaptic to the giant fiber (the tergotrochanteral muscle motor neuron, TTMn (A

https://doi.org/10.7554/eLife.34272.021
Figure 5 with 6 supplements
Distinguishing DN inputs and outputs.

(A) The morphology of DNp02. The DN neurites have a smooth appearance in the posterior ventral lateral protocerebrum (PVLP) and anterior mechanosensory motor center (AMMC), and varicose processes in …

https://doi.org/10.7554/eLife.34272.022
Figure 5—figure supplement 1
DN presynaptic terminals in the gnathal ganglion.

DN innervation in the brain (A1–J1) and VNC (A2–J2) obtained with the multicolor flip-out technique, and the corresponding result of synaptotagmin labeling in the GNG (A3–J3). Ten examples of DNs …

https://doi.org/10.7554/eLife.34272.023
Figure 5—figure supplement 2
DN presynaptic terminals in the brain.

The result of synaptotagmin labeling of split-GAL4 lines targeting four DN types with prominent varicose endings in the brain. Insets show magnification of regions indicated by orange dotted lines. …

https://doi.org/10.7554/eLife.34272.024
Figure 5—figure supplement 3
DNs in the pars intercerebralis.

(A–D) Morphology of DNc01 and c02. Whole morphology (A, C) and horizontal views of VNC (B, D) are shown. Both cell types have their cell body located in the pars intercerebralis, where Hsu and …

https://doi.org/10.7554/eLife.34272.025
Figure 5—figure supplement 4
DN presynaptic terminals in the brain.

(A) Synaptotagmin labeling of DNp04 (green, membrane-bound GFP; magenta, synaptotagmin), shown as a maximum intensity projection of the whole morphology in the brain. Inset shows magnification of …

https://doi.org/10.7554/eLife.34272.026
Figure 5—figure supplement 5
Determining polarity of DNa05 by cell-specific co-expression of a membrane-bound GFP (green) and thepresynaptic reporter synaptotagmin-smGFP-HA (magenta).

Images of the brain (A) and VNC (B) are shown.

https://doi.org/10.7554/eLife.34272.027
Figure 5—figure supplement 6
Reproducibility of neuronal labeling in Split-Gal4 lines.

Three samples are shown for DNp10 (SS01580), DNg07 (SS01074) and DNp01 (SS00727).

https://doi.org/10.7554/eLife.34272.028
Figure 6 with 1 supplement
The number of identified DNs and their neurite distribution.

(A) The number of DNs innervating individual brain regions with smooth process (top) and varicose process (bottom). The inset is a heat map of DN innervation in the brain: sagittal and frontal views …

https://doi.org/10.7554/eLife.34272.029
Figure 6—figure supplement 1
Laterality and extent of DNs axonal projections.

(A–C) The number of DN types innervating individual brain regions (A), tracts (B), and VNC regions (C) divided into ipsilateral- (green) and contralateral- (magenta) projecting DNs. (D) The number …

https://doi.org/10.7554/eLife.34272.030
Figure 7 with 4 supplements
DNs differentially address wing/neck/haltere and leg motor systems.

(A) Clustering of VNC neuropils based on patterns of innervation by each DN. Filled pixels indicate that we observed varicose processes in the neuropil represented by the corresponding row, for the …

https://doi.org/10.7554/eLife.34272.031
Figure 7—figure supplement 1
Putative octopaminergic DNs.

(A–B) Morphology of DNd02. Whole cell morphology (A) and frontal sections of VNC (B) are shown. The cell body is located in the GNG and the axon descends ipsilaterally through tract DLV. d02 has …

https://doi.org/10.7554/eLife.34272.032
Figure 7—figure supplement 2
DN projecting to both wing and leg neuropil.

(A) DNg17 has smooth process in the saddle and GNG, and varicose process in the GNG. The DN descends contralaterally in the neck connective and projects to both leg and wing neuropils. (B,C) …

https://doi.org/10.7554/eLife.34272.033
Figure 7—figure supplement 3
DN projecting to the ventral association center.

(A) Morphology of DNg20. The DN has smooth processes in the saddle and GNG, and smooth and varicose processes in the GNG. The cell body is located on the GNG and the DN descends the contralateral …

https://doi.org/10.7554/eLife.34272.034
Figure 7—figure supplement 4
DN projecting outside the VNC.

(A) Lateral view of DNg28 morphology. The DN has smooth processes in the GNG, and varicose processes in the nerve fiber. The axon mainly runs through the dorsal surface of the VNC. (B) Four examples …

https://doi.org/10.7554/eLife.34272.035
Brain innervation by DNs.

(A) Clustering of brain neuropils based on patterns of DN innervation. Both brain neuropils (rows) and DNs (columns) were sorted by hierarchical clustering based on Pearson’s correlation as a metric …

https://doi.org/10.7554/eLife.34272.036
Figure 9 with 3 supplements
DN connectivity between the brain and VNC.

(A) Dendritic distribution of DNs grouped by output. Heat map colors indicate the number of DN types innervating each brain neuropil for different groups of DNs (A1–A8) defined by their projection …

https://doi.org/10.7554/eLife.34272.037
Figure 9—figure supplement 1
Innervation profile of DNs sorted by innervation clusters in the VNC.

In each row, a filled pixel indicates the presence of innervation by the corresponding DN in the corresponding CNS neuropil column. Smooth process in the brain and varicose endings in the VNC are …

https://doi.org/10.7554/eLife.34272.038
Figure 9—figure supplement 2
Innervation profile of DNs sorted by innervation clusters in the brain.

In each row, a filled pixel indicates the presence of innervation by the corresponding DN in the corresponding CNS neuropil column. Smooth process in the brain and varicose endings in the VNC are …

https://doi.org/10.7554/eLife.34272.039
Figure 9—figure supplement 3
Neuronal pathways of DNs.

(A) Location of the DN tracts. Horizontal view of the whole VNC (left) and frontal sections of each segment are shown (right). Each frontal plane corresponds to the depth shown in dotted lines in …

https://doi.org/10.7554/eLife.34272.040
Figure 10 with 2 supplements
Tract-based analysis of DN axonal projection patterns in leg neuropils.

(A) Major DN types projecting to leg neuropils have different terminal patterns that segregate by descending tract. Horizontal (A1) and frontal (A2) views of overlaid aligned DNs running through …

https://doi.org/10.7554/eLife.34272.041
Figure 10—figure supplement 1
DNs running through the oblique tract.

(A) Morphologies of six DNs with axons in the oblique tract. (B–D) Merged horizontal (B), sagittal (C), and frontal (D) views of the six DNs shown in panel (A), showing overlap and formation of the …

https://doi.org/10.7554/eLife.34272.042
Figure 10—figure supplement 2
Potential overlap between DNs and leg motor neurons.

(A) Leg motor neuron dendrites form a myotopic map in each leg neuropil. Dendritic regions for motor neurons corresponding to different leg segments are schematized as different colored regions, …

https://doi.org/10.7554/eLife.34272.043
Figure 11 with 2 supplements
Tract-based analysis of DN axonal projection patterns in the wing neuropil.

(A) Sagittal view of two example DN types targeting wing neuropil (blue dashed line) via the MTD tract. The axon of DNp03 (green) travels ventrally, with the volume of its major axon in the MTD. In …

https://doi.org/10.7554/eLife.34272.044
Figure 11—figure supplement 1
Innervation in the wing neuropil of DNs and interneurons.

(A,B) Confocal stacks of power muscle (green) and steering muscle (magenta) motor neurons (MNs). Horizontal (A) and frontal (B) views are shown. For each image, the depth of the horizontal plane is …

https://doi.org/10.7554/eLife.34272.045
Figure 11—figure supplement 2
Innervation in the wing neuropil of DNs and interneurons.

(A) Reconstructed morphology of four different DNs running through the dorsal route of the MTD shown with pseudo-color for depth. Cold and hot colors indicate the dorsal and ventral part of the wing …

https://doi.org/10.7554/eLife.34272.046
Figure 12 with 3 supplements
DNs from optic glomeruli.

(A) A matrix indicating DN innervation in the optic glomeruli. Neurite innervation in individual glomeruli was observed with a 63x objective. Black and gray pixel shading represent dense and sparse …

https://doi.org/10.7554/eLife.34272.047
Figure 12—figure supplement 1
Morphology of DNs from optic glomeruli in the posterior ventral protocerebrum.

High-resolution (63x) confocal images of the 10 different DNs innervating the optic glomeruli in the PVLP. Neurite morphology in the brain is shown, as well as images at the depth of the optic …

https://doi.org/10.7554/eLife.34272.048
Figure 12—figure supplement 2
Morphology of DNs from optic glomeruli in the posterior lateral protocerebrum.

High resolution (63x) confocal images of the six different DNs innervating the PLP optic glomeruli. Images as in Figure 10. The majority of these DNs (5 out of 6) descend contralaterally through the …

https://doi.org/10.7554/eLife.34272.049
Figure 12—figure supplement 3
Morphology of a DN innervating both olfactory and optic glomeruli.

(A) Morphology of DNb05. (B) Segmentation of DNb05 on both sides of the CNS. The outer shapes of the brain and VNC are shown with gray. (C) Reconstruction of DN innervation in the antennal lobe. …

https://doi.org/10.7554/eLife.34272.050
DNs forming a dendritic cluster within the LC4 glomerulus project to the lower tectulum.

(A) The morphologies of DNs which have dendritic innervation to the LC4 glomerulus (‘LC4 DNs’). The maximum intensity projection of a confocal stack with 20x objective are shown. All DNs partially …

https://doi.org/10.7554/eLife.34272.051
Anatomical organization of DNs in Drosophila.

(A) Sagittal view schematic of brain and VNC illustrating the major descending pathways. Gross innervation areas of different DN types are shown with color. Inset shows number of each DN type …

https://doi.org/10.7554/eLife.34272.052
Biased representation in the protocerebrum among DNs targeting wing and leg motor areas.

(A) Morphology of DNs preferentially innervating anterior (DNg02, right) and posterior parts of the brain (DNg13, left). DNg02 projects to dorsal VNC, whereas DNg13 projects to ventral VNC. (B–C) …

https://doi.org/10.7554/eLife.34272.053

Tables

Key resources table
Reagent type (species)
or resource
DesignationSource or referenceIdentifiersAdditional information
Genetic reagent
(Drosophila melanogaster)
VT040698-x-VT025718this papersplit_gal4.janelia.org:SS02536split-GAL4 driver line targeting DNa05
Genetic reagent
(Drosophila melanogaster)
VT040698-x-VT028606this papersplit_gal4.janelia.org:SS01546split-GAL4 driver line targeting DNa05,
DNa07
Genetic reagent
(Drosophila melanogaster)
R31H10-x-VT040698this papersplit_gal4.janelia.org:SS01560split-GAL4 driver line targeting DNa07
Genetic reagent
(Drosophila melanogaster)
VT023490-x-R80H02this papersplit_gal4.janelia.org:SS02393split-GAL4 driver line targeting DNa08
Genetic reagent
(Drosophila melanogaster)
R55D12-x-R72H04this papersplit_gal4.janelia.org:SS02370split-GAL4 driver line targeting DNb03
Genetic reagent
(Drosophila melanogaster)
R55D12-x-VT063306this papersplit_gal4.janelia.org:SS02552split-GAL4 driver line targeting DNb03
Genetic reagent
(Drosophila melanogaster)
VT019391-x-VT028198this papersplit_gal4.janelia.org:SS01051split-GAL4 driver line targeting DNb05
Genetic reagent
(Drosophila melanogaster)
R20C04-x-VT025999this papersplit_gal4.janelia.org:SS02631split-GAL4 driver line targeting DNb06
Genetic reagent
(Drosophila melanogaster)
R59A06-x-R64B11this papersplit_gal4.janelia.org:SS04161split-GAL4 driver line targeting DNc01
Genetic reagent
(Drosophila melanogaster)
VT012639-x-VT034795this papersplit_gal4.janelia.org:SS01570split-GAL4 driver line targeting DNd02,
DNd03
Genetic reagent
(Drosophila melanogaster)
VT059225-x-VT057283this papersplit_gal4.janelia.org:SS02111split-GAL4 driver line targeting DNg10
Genetic reagent
(Drosophila melanogaster)
VT049363-x-VT057283this papersplit_gal4.janelia.org:SS01547split-GAL4 driver line targeting DNg10
Genetic reagent
(Drosophila melanogaster)
VT037583-x-R81C11this papersplit_gal4.janelia.org:SS01579split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
VT037825-x-VT020379this papersplit_gal4.janelia.org:SS02538split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
VT059450-x-VT043626this papersplit_gal4.janelia.org:SS02285split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
R38H06-x-VT018689this papersplit_gal4.janelia.org:SS01069split-GAL4 driver line targeting DNg14
Genetic reagent
(Drosophila melanogaster)
R53D12-x-VT018689this papersplit_gal4.janelia.org:SS04158split-GAL4 driver line targeting DNg14
Genetic reagent
(Drosophila melanogaster)
VT020033-x-VT008483this papersplit_gal4.janelia.org:SS02279split-GAL4 driver line targeting DNg17
Genetic reagent
(Drosophila melanogaster)
VT020033-x-VT014208this papersplit_gal4.janelia.org:SS00898split-GAL4 driver line targeting DNg17
Genetic reagent
(Drosophila melanogaster)
VT039465-x-VT023750this papersplit_gal4.janelia.org:SS02625split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
VT048835-x-R24C07this papersplit_gal4.janelia.org:SS01046split-GAL4 driver line targeting DNp24
Genetic reagent
(Drosophila melanogaster)
VT056582-x-R22D06this papersplit_gal4.janelia.org:SS01052split-GAL4 driver line targeting DNp25
Genetic reagent
(Drosophila melanogaster)
R21F01-x-R22D06this papersplit_gal4.janelia.org:SS01059split-GAL4 driver line targeting DNp25
Genetic reagent
(Drosophila melanogaster)
R27E07-x-VT014958this papersplit_gal4.janelia.org:SS01558split-GAL4 driver line targeting DNg26
Genetic reagent
(Drosophila melanogaster)
R13D04-x-R65D05this papersplit_gal4.janelia.org:SS01557split-GAL4 driver line targeting DNg27
Genetic reagent
(Drosophila melanogaster)
R59F08-x-R47H03this papersplit_gal4.janelia.org:SS04159split-GAL4 driver line targeting DNg29
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT005005this papersplit_gal4.janelia.org:SS02548split-GAL4 driver line targeting DNg03
Genetic reagent
(Drosophila melanogaster)
R61A01-x-R13B05this papersplit_gal4.janelia.org:SS02378split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R14H09-x-R61A01this papersplit_gal4.janelia.org:SS02388split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R14H09-x-R56H09this papersplit_gal4.janelia.org:SS01054split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R61A01-x-VT057280this papersplit_gal4.janelia.org:SS01077split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R14H09-x-VT032228this papersplit_gal4.janelia.org:SS02316split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT042734this papersplit_gal4.janelia.org:SS02324split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R42B02-x-R89B01this papersplit_gal4.janelia.org:SS01074split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT026005this papersplit_gal4.janelia.org:SS01597split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT059427this papersplit_gal4.janelia.org:SS02547split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R73A05-x-VT059427this papersplit_gal4.janelia.org:SS02554split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT037574this papersplit_gal4.janelia.org:SS02635split-GAL4 driver line targeting DNg07,
DNg08
Genetic reagent
(Drosophila melanogaster)
R10A12-x-VT004455this papersplit_gal4.janelia.org:SS02299split-GAL4 driver line targeting DNp01
Genetic reagent
(Drosophila melanogaster)
R94E01-x-VT031084this papersplit_gal4.janelia.org:SS01608split-GAL4 driver line targeting DNp10
Genetic reagent
(Drosophila melanogaster)
R94E01-x-R48E11this papersplit_gal4.janelia.org:SS02385split-GAL4 driver line targeting DNp10
Genetic reagent
(Drosophila melanogaster)
VT017683-x-VT031084this papersplit_gal4.janelia.org:SS01049split-GAL4 driver line targeting DNp10
Genetic reagent
(Drosophila melanogaster)
VT031084-x-R48E11this papersplit_gal4.janelia.org:SS01580split-GAL4 driver line targeting DNp10
Genetic reagent
(Drosophila melanogaster)
R69C11-x-R81D05this papersplit_gal4.janelia.org:SS01078split-GAL4 driver line targeting DNp20
Genetic reagent
(Drosophila melanogaster)
R17A04-x-R24A03this papersplit_gal4.janelia.org:SS01056split-GAL4 driver line targeting DNp28
Genetic reagent
(Drosophila melanogaster)
R20C03-x-R23C07this papersplit_gal4.janelia.org:SS01589split-GAL4 driver line targeting DNp28
Genetic reagent
(Drosophila melanogaster)
R11H10-x-VT040348this papersplit_gal4.janelia.org:SS02618split-GAL4 driver line targeting DNp29
Genetic reagent
(Drosophila melanogaster)
R29F12-x-R37G07this papersplit_gal4.janelia.org:SS01596split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R60B12-x-R58E05this papersplit_gal4.janelia.org:SS02387split-GAL4 driver line targeting DNg30
Genetic reagent
(Drosophila melanogaster)
R84B12-x-VT048835this papersplit_gal4.janelia.org:SS01080split-GAL4 driver line targeting DNp04
Genetic reagent
(Drosophila melanogaster)
VT048835-x-VT017647this papersplit_gal4.janelia.org:SS01544split-GAL4 driver line targeting DNp04,
DNp02
Genetic reagent
(Drosophila melanogaster)
VT017411-x-VT017647this papersplit_gal4.janelia.org:SS02292split-GAL4 driver line targeting DNp04,
DNp06
Genetic reagent
(Drosophila melanogaster)
VT019018-x-VT017411this papersplit_gal4.janelia.org:SS02256split-GAL4 driver line targeting DNp06
Genetic reagent
(Drosophila melanogaster)
VT029814-x-VT047755this papersplit_gal4.janelia.org:SS02276split-GAL4 driver line targeting DNp07
Genetic reagent
(Drosophila melanogaster)
VT023490-x-R38F04this papersplit_gal4.janelia.org:SS01540split-GAL4 driver line targeting DNp09
Genetic reagent
(Drosophila melanogaster)
VT047755-x-VT025718this papersplit_gal4.janelia.org:SS02310split-GAL4 driver line targeting several
types of DNa DNs
Genetic reagent
(Drosophila melanogaster)
R22C05-x-R56G08this papersplit_gal4.janelia.org:SS00731split-GAL4 driver line targeting DNa01
Genetic reagent
(Drosophila melanogaster)
R75C10-x-R87D07this papersplit_gal4.janelia.org:SS00730split-GAL4 driver line targeting DNa02
Genetic reagent
(Drosophila melanogaster)
VT047755-x-R47D05this papersplit_gal4.janelia.org:SS02384split-GAL4 driver line targeting DNa04,
DNa10
Genetic reagent
(Drosophila melanogaster)
VT061919-x-VT040698this papersplit_gal4.janelia.org:SS01552split-GAL4 driver line targeting DNa05,
DNa07
Genetic reagent
(Drosophila melanogaster)
VT048835-x-VT017682this papersplit_gal4.janelia.org:SS01572split-GAL4 driver line targeting DNa05,
DNp11
Genetic reagent
(Drosophila melanogaster)
VT028606-x-R56G08this papersplit_gal4.janelia.org:SS01541split-GAL4 driver line targeting DNa07
Genetic reagent
(Drosophila melanogaster)
VT028606-x-R87B09this papersplit_gal4.janelia.org:SS01542split-GAL4 driver line targeting DNa07
Genetic reagent
(Drosophila melanogaster)
VT028606-x-VT008675this papersplit_gal4.janelia.org:SS01571split-GAL4 driver line targeting DNa07
Genetic reagent
(Drosophila melanogaster)
VT013121-x-R45H03this papersplit_gal4.janelia.org:SS02383split-GAL4 driver line targeting DNb01
Genetic reagent
(Drosophila melanogaster)
VT049749-x-VT013121this papersplit_gal4.janelia.org:SS02542split-GAL4 driver line targeting DNb01
Genetic reagent
(Drosophila melanogaster)
R21F05-x-R93B10this papersplit_gal4.janelia.org:SS02396split-GAL4 driver line targeting DNb02
Genetic reagent
(Drosophila melanogaster)
R59B10-x-R21F05this papersplit_gal4.janelia.org:SS01600split-GAL4 driver line targeting DNb02
Genetic reagent
(Drosophila melanogaster)
R21F05-x-R21H11this papersplit_gal4.janelia.org:SS01060split-GAL4 driver line targeting DNb02
Genetic reagent
(Drosophila melanogaster)
VT027175-x-R83B06this papersplit_gal4.janelia.org:SS02395split-GAL4 driver line targeting DNc02
Genetic reagent
(Drosophila melanogaster)
VT001615-x-VT043132this papersplit_gal4.janelia.org:SS01545split-GAL4 driver line targeting DNd02
Genetic reagent
(Drosophila melanogaster)
VT001615-x-VT026017this papersplit_gal4.janelia.org:SS01575split-GAL4 driver line targeting DNd02
Genetic reagent
(Drosophila melanogaster)
VT012639-x-VT045791this papersplit_gal4.janelia.org:SS01569split-GAL4 driver line targeting DNd02,
DNd03
Genetic reagent
(Drosophila melanogaster)
VT001615-x-R70C05this papersplit_gal4.janelia.org:SS01576split-GAL4 driver line targeting DNd02,
DNd03
Genetic reagent
(Drosophila melanogaster)
VT049363-x-VT059225this papersplit_gal4.janelia.org:SS02278split-GAL4 driver line targeting DNg10
Genetic reagent
(Drosophila melanogaster)
R81C11-x-R66B05this papersplit_gal4.janelia.org:SS02391split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
VT037583-x-VT025598this papersplit_gal4.janelia.org:SS01550split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
R66B05-x-R85H06this papersplit_gal4.janelia.org:SS01566split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
VT037574-x-VT025598this papersplit_gal4.janelia.org:SS02617split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
VT025739-x-VT032280this papersplit_gal4.janelia.org:SS02609split-GAL4 driver line targeting DNg12
Genetic reagent
(Drosophila melanogaster)
VT025739-x-VT025999this papersplit_gal4.janelia.org:SS02608split-GAL4 driver line targeting DNg12
Genetic reagent
(Drosophila melanogaster)
VT027166-x-VT009857this papersplit_gal4.janelia.org:SS02259split-GAL4 driver line targeting DNg13
Genetic reagent
(Drosophila melanogaster)
R88F03-x-VT009857this papersplit_gal4.janelia.org:SS01567split-GAL4 driver line targeting DNg13
Genetic reagent
(Drosophila melanogaster)
VT047747-x-VT037825this papersplit_gal4.janelia.org:SS02275split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
R60B11-x-R83B04this papersplit_gal4.janelia.org:SS01565split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
VT043400-x-VT043662this papersplit_gal4.janelia.org:SS02377split-GAL4 driver line targeting DNg15
Genetic reagent
(Drosophila melanogaster)
VT043288-x-VT028153this papersplit_gal4.janelia.org:SS01543split-GAL4 driver line targeting DNg16
Genetic reagent
(Drosophila melanogaster)
VT023750-x-VT039465this papersplit_gal4.janelia.org:SS02624split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT042835this papersplit_gal4.janelia.org:SS02634split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
R42B02-x-R65C10this papersplit_gal4.janelia.org:SS01073split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT011148this papersplit_gal4.janelia.org:SS01561split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
R14F03-x-R24C07this papersplit_gal4.janelia.org:SS00732split-GAL4 driver line targeting DNp24
Genetic reagent
(Drosophila melanogaster)
R64B03-x-R29G08this papersplit_gal4.janelia.org:SS01602split-GAL4 driver line targeting DNg25
Genetic reagent
(Drosophila melanogaster)
VT057470-x-VT033947this papersplit_gal4.janelia.org:SS00923split-GAL4 driver line targeting DNp27
Genetic reagent
(Drosophila melanogaster)
R10A07-x-VT057470this papersplit_gal4.janelia.org:SS02257split-GAL4 driver line targeting DNp27
Genetic reagent
(Drosophila melanogaster)
R22H02-x-R20F03this papersplit_gal4.janelia.org:SS01061split-GAL4 driver line targeting DNg26
Genetic reagent
(Drosophila melanogaster)
R27E07-x-R14D01this papersplit_gal4.janelia.org:SS01064split-GAL4 driver line targeting DNg26
Genetic reagent
(Drosophila melanogaster)
R27E07-x-R20F03this papersplit_gal4.janelia.org:SS01593split-GAL4 driver line targeting DNg26
Genetic reagent
(Drosophila melanogaster)
R24F06-x-R45E06this papersplit_gal4.janelia.org:SS01063split-GAL4 driver line targeting DNg28
Genetic reagent
(Drosophila melanogaster)
R44A07-x-VT045150this papersplit_gal4.janelia.org:SS01564split-GAL4 driver line targeting DNg28
Genetic reagent
(Drosophila melanogaster)
R47H03-x-R59F08this papersplit_gal4.janelia.org:SS01075split-GAL4 driver line targeting DNg29
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT044958this papersplit_gal4.janelia.org:SS02633split-GAL4 driver line targeting DNg03
Genetic reagent
(Drosophila melanogaster)
R14H09-x-R13B05this papersplit_gal4.janelia.org:SS01588split-GAL4 driver line targeting DNp26
genetic reagent
(Drosophila melanogaster)
VT003476-x-R89A03this papersplit_gal4.janelia.org:SS01553split-GAL4 driver line targeting DNp26
Genetic reagent
(Drosophila melanogaster)
R73A05-x-VT012287this papersplit_gal4.janelia.org:SS01604split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R14A01-x-R79H02this papersplit_gal4.janelia.org:SS00727split-GAL4 driver line targeting DNp01
Genetic reagent
(Drosophila melanogaster)
R25C08-x-R68A06this papersplit_gal4.janelia.org:SS00726split-GAL4 driver line targeting DNp01
Genetic reagent
(Drosophila melanogaster)
VT025392-x-VT057247this papersplit_gal4.janelia.org:SS02891split-GAL4 driver line targeting DNp11
Genetic reagent
(Drosophila melanogaster)
R11E07-x-R77F05this papersplit_gal4.janelia.org:SS01556split-GAL4 driver line targeting DNp15
Genetic reagent
(Drosophila melanogaster)
R49A07-x-R55A03this papersplit_gal4.janelia.org:SS00735split-GAL4 driver line targeting DNp16
Genetic reagent
(Drosophila melanogaster)
R67E08-x-VT025789this papersplit_gal4.janelia.org:SS02553split-GAL4 driver line targeting DNp17
Genetic reagent
(Drosophila melanogaster)
VT064490-x-R69C11this papersplit_gal4.janelia.org:SS02392split-GAL4 driver line targeting DNp18
Genetic reagent
(Drosophila melanogaster)
VT063736-x-R24A03this papersplit_gal4.janelia.org:SS01053split-GAL4 driver line targeting DNp02
Genetic reagent
(Drosophila melanogaster)
VT063736-x-VT017647this papersplit_gal4.janelia.org:SS01554split-GAL4 driver line targeting DNp02
Genetic reagent
(Drosophila melanogaster)
VT025392-x-R15E12this papersplit_gal4.janelia.org:SS02379split-GAL4 driver line targeting DNp02,
DNp11
Genetic reagent
(Drosophila melanogaster)
VT025392-x-VT017647this papersplit_gal4.janelia.org:SS02534split-GAL4 driver line targeting DNp02,
DNp11
Genetic reagent
(Drosophila melanogaster)
R58G11-x-R81D05this papersplit_gal4.janelia.org:SS00729split-GAL4 driver line targeting DNp20
Genetic reagent
(Drosophila melanogaster)
R20C05-x-R85H06this papersplit_gal4.janelia.org:SS01057split-GAL4 driver line targeting DNp20
Genetic reagent
(Drosophila melanogaster)
R20C03-x-R31B08this papersplit_gal4.janelia.org:SS01590split-GAL4 driver line targeting DNp28
Genetic reagent
(Drosophila melanogaster)
R11H10-x-VT033947this papersplit_gal4.janelia.org:SS01587split-GAL4 driver line targeting DNp29
Genetic reagent
(Drosophila melanogaster)
R30C12-x-R22D06this papersplit_gal4.janelia.org:SS01066split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R91C05-x-R31B08this papersplit_gal4.janelia.org:SS01081split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R29F12-x-R88C07this papersplit_gal4.janelia.org:SS01559split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R91C05-x-R37G07this papersplit_gal4.janelia.org:SS02382split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R61H01-x-R82C10this papersplit_gal4.janelia.org:SS02394split-GAL4 driver line targeting DNp32
Genetic reagent
(Drosophila melanogaster)
VT032898-x-VT048835this papersplit_gal4.janelia.org:SS00934split-GAL4 driver line targeting DNp04
Genetic reagent
(Drosophila melanogaster)
R50D07-x-R33H11this papersplit_gal4.janelia.org:SS00725split-GAL4 driver line targeting DNp04
Genetic reagent
(Drosophila melanogaster)
VT019060-x-VT003280this papersplit_gal4.janelia.org:SS00865split-GAL4 driver line targeting DNp05
Genetic reagent
(Drosophila melanogaster)
VT019018-x-VT017647this papersplit_gal4.janelia.org:SS01047split-GAL4 driver line targeting DNp06
Genetic reagent
(Drosophila melanogaster)
VT047755-x-VT003280this papersplit_gal4.janelia.org:SS02612split-GAL4 driver line targeting DNp07
Genetic reagent
(Drosophila melanogaster)
VT029814-x-VT003280this papersplit_gal4.janelia.org:SS01549split-GAL4 driver line targeting DNp07,
DNp13
Genetic reagent
(Drosophila melanogaster)
VT032900-x-VT043145this papersplit_gal4.janelia.org:SS01582split-GAL4 driver line targeting DNb04
Genetic reagent
(Drosophila melanogaster)
VT008142-x-VT046808this papersplit_gal4.janelia.org:SS01581split-GAL4 driver line targeting DNp13,
DNp30
Genetic reagent
(Drosophila melanogaster)
R24A03-x-R74C01this papersplit_gal4.janelia.org:SS01062split-GAL4 driver line empty
brain control
Genetic reagent
(Drosophila melanogaster)
R19G08-x-R47F01this papersplit_gal4.janelia.org:SS04528split-GAL4 driver line targeting motor
neuron b1 (mnb1), a VNC interneuron,
with weak expression in DNg02
and possibly DNg06
Genetic reagent
(Drosophila melanogaster)
R10A12-x-R81E05this papersplit_gal4.janelia.org:SS00737split-GAL4 driver line targeting DNp20,
DNp22, motor neuron
b2 (mnb2), and dorsoventral muscle
motor neurons (DVM)
Genetic reagent
(Drosophila melanogaster)
R18A05-x-VT045791this papersplit_gal4.janelia.org:SS31976split-GAL4 driver line targeting
an interneuron
Genetic reagent
(Drosophila melanogaster)
VT016973-x-VT002042this papersplit_gal4.janelia.org:SS02623split-GAL4 driver line targeting
the tergotrochanteral muscle
motor neuron (TTMn)
Genetic reagent
(Drosophila melanogaster)
VT049105doi: 10.1101/198648flweb.janelia.org:VT049105split-GAL4 driver line targeting
the peripherally synapsing interneuron
(PSI) and other VNC interneurons
Genetic reagent
(Drosophila melanogaster)
VT007170-x-VT041658this papersplit_gal4.janelia.org:SS02628split-GAL4 driver line targeting
an interneuron
Genetic reagent
(Drosophila melanogaster)
R26E02-x-R81D05this papersplit_gal4.janelia.org:SS01592split-GAL4 driver line targeting DNp18,
DNp22, and the motor
neuron for wing steering
muscle III1 (III1mn)
Genetic reagent
(Drosophila melanogaster)
R31B08-x-R24A03this papersplit_gal4.janelia.org:SS00724split-GAL4 driver line targeting DNp03,
DNp28, DNp02
Genetic reagent
(Drosophila melanogaster)
R25C08-x-R79H02this papersplit_gal4.janelia.org:SS00728split-GAL4 driver line targeting DNp01
Genetic reagent
(Drosophila melanogaster)
R58E07-x-R39H12this papersplit_gal4.janelia.org:SS00733split-GAL4 driver line targeting DNp31
Genetic reagent
(Drosophila melanogaster)
R73C04-x-R39H12this papersplit_gal4.janelia.org:SS00734split-GAL4 driver line targeting DNb05
Genetic reagent
(Drosophila melanogaster)
R21A07-x-R72A01this papersplit_gal4.janelia.org:SS00736split-GAL4 driver line targeting DNa05
Genetic reagent
(Drosophila melanogaster)
R81D05-x-R81E05this papersplit_gal4.janelia.org:SS00738split-GAL4 driver line targeting
DNp03, DNp18, DNp20, DNp22
Genetic reagent
(Drosophila melanogaster)
VT040541-x-VT050661this papersplit_gal4.janelia.org:SS01048split-GAL4 driver line targeting DNd01
Genetic reagent
(Drosophila melanogaster)
R21C05-x-R28E01this papersplit_gal4.janelia.org:SS01058split-GAL4 driver line targeting DNg09
Genetic reagent
(Drosophila melanogaster)
R30C01-x-R85H01this papersplit_gal4.janelia.org:SS01065split-GAL4 driver line targeting DNp04
Genetic reagent
(Drosophila melanogaster)
R31B08-x-R88C07this papersplit_gal4.janelia.org:SS01067split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R32C05-x-R70C05this papersplit_gal4.janelia.org:SS01068split-GAL4 driver line
targeting DNd02, DNd03
Genetic reagent
(Drosophila melanogaster)
R38H06-x-R65D06this papersplit_gal4.janelia.org:SS01070split-GAL4 driver line targeting DNa10
Genetic reagent
(Drosophila melanogaster)
R40H12-x-R85H01this papersplit_gal4.janelia.org:SS01071split-GAL4 driver line targeting DNp04,
DNp20
Genetic reagent
(Drosophila melanogaster)
R42B02-x-R65C02this papersplit_gal4.janelia.org:SS01072split-GAL4 driver line targeting DNg07
Genetic reagent
(Drosophila melanogaster)
R50D07-x-R74C01this papersplit_gal4.janelia.org:SS01076split-GAL4 driver line targeting DNp04
Genetic reagent
(Drosophila melanogaster)
VT059450-x-VT037825this papersplit_gal4.janelia.org:SS01551split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
R10A07-x-VT033947this papersplit_gal4.janelia.org:SS01555split-GAL4 driver line targeting DNp27
Genetic reagent
(Drosophila melanogaster)
R91C05-x-R10A12this papersplit_gal4.janelia.org:SS01568split-GAL4 driver line targeting DNp20
Genetic reagent
(Drosophila melanogaster)
VT045148-x-VT045150this papersplit_gal4.janelia.org:SS01574split-GAL4 driver line targeting DNg28
Genetic reagent
(Drosophila melanogaster)
VT004985-x-VT017928this papersplit_gal4.janelia.org:SS01583split-GAL4 driver line targeting DNp11,
DNp28
Genetic reagent
(Drosophila melanogaster)
VT034258-x-R32B03this papersplit_gal4.janelia.org:SS01584split-GAL4 driver line targeting DNg15
Genetic reagent
(Drosophila melanogaster)
R10A07-x-R24D07this papersplit_gal4.janelia.org:SS01585split-GAL4 driver line targeting DNp27
Genetic reagent
(Drosophila melanogaster)
R29F12-x-R24A03this papersplit_gal4.janelia.org:SS01594split-GAL4 driver line targeting DNp02,
DNp03, DNp28
Genetic reagent
(Drosophila melanogaster)
R29F12-x-R31B08this papersplit_gal4.janelia.org:SS01595split-GAL4 driver line targeting DNp03
Genetic reagent
(Drosophila melanogaster)
R42B02-x-VT015782this papersplit_gal4.janelia.org:SS01598split-GAL4 driver line targeting DNg06
Genetic reagent
(Drosophila melanogaster)
R47D05-x-R39H12this papersplit_gal4.janelia.org:SS01599split-GAL4 driver line targeting DNa04
Genetic reagent
(Drosophila melanogaster)
R59B10-x-R21H11this papersplit_gal4.janelia.org:SS01601split-GAL4 driver line targeting DNb02
Genetic reagent
(Drosophila melanogaster)
R72H09-x-VT037825this papersplit_gal4.janelia.org:SS01603split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
R77H03-x-R74B04this papersplit_gal4.janelia.org:SS01605split-GAL4 driver line targeting DNb01
Genetic reagent
(Drosophila melanogaster)
VT015782-x-VT061933this papersplit_gal4.janelia.org:SS02260split-GAL4 driver line targeting DNg06
Genetic reagent
(Drosophila melanogaster)
VT020379-x-VT043626this papersplit_gal4.janelia.org:SS02541split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
R22C05-x-VT025718this papersplit_gal4.janelia.org:SS02545split-GAL4 driver line targeting DNa02
Genetic reagent
(Drosophila melanogaster)
R81C11-x-VT025598this papersplit_gal4.janelia.org:SS02555split-GAL4 driver line targeting DNg11
Genetic reagent
(Drosophila melanogaster)
VT029814-x-VT008145this papersplit_gal4.janelia.org:SS02610split-GAL4 driver line targeting DNp13
Genetic reagent
(Drosophila melanogaster)
R58E07-x-R30C01this papersplit_gal4.janelia.org:SS02621split-GAL4 driver line targeting DNp31
Genetic reagent
(Drosophila melanogaster)
R38H06-x-R50B07this papersplit_gal4.janelia.org:SS04160split-GAL4 driver line targeting DNg14
Genetic reagent
(Drosophila melanogaster)
R40F04-x-R83B06this papersplit_gal4.janelia.org:SS04530split-GAL4 driver line targeting DNc01,
DNc02
Genetic reagent
(Drosophila melanogaster)
VT042835-x-R65C10this papersplit_gal4.janelia.org:SS05089split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
R44A07-x-VT062604this papersplit_gal4.janelia.org:SS05099split-GAL4 driver line targeting DNg28
Genetic reagent
(Drosophila melanogaster)
VT023750-x-VT042835this papersplit_gal4.janelia.org:SS05107split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
VT042835-x-VT023750this papersplit_gal4.janelia.org:SS05116split-GAL4 driver line targeting DNg02
Genetic reagent
(Drosophila melanogaster)
VT042835-x-VT020379this papersplit_gal4.janelia.org:SS05122split-GAL4 driver line targeting DNg01
Genetic reagent
(Drosophila melanogaster)
pJFRC51-3xUAS-Syt::
smGFP-HA in
su(Hw)attPa
Aso et al., 2014
Genetic reagent
(Drosophila melanogaster)
pJFRC22-10XUAS-
IVS-myr::tdTomato
Pfeiffer et al. 2010https://www.janelia.org/lab/rubin-lab/tools-reagents
Genetic reagent
(Drosophila melanogaster)
pJFRC93-13XLexAop2-
IVS-Syn21-mPA-p10
(VK00005)
Pfeiffer et al. 2012https://www.janelia.org/lab/rubin-lab/tools-reagents
Genetic reagent
(Drosophila melanogaster)
nsyb-LexAp65
(attP2)/ TM2
Rubin, G.Personal communicaiton
Genetic reagent
(Drosophila melanogaster)
mPA-LexAopPfeiffer et al., 2012
Genetic reagent
(Drosophila melanogaster)
tsh-LexASimpson, 2016
Genetic reagent
(Drosophila melanogaster)
LexAop2-GAL80Pfeiffer, B.http://flybase.org/reports/FBrf0212441
Genetic reagent
(Drosophila melanogaster)
pJFRC20-8XLexAop2-
IVS-GAL80-WPRE
(su(Hw)attP5)
Pfeiffer et al., 2010https://www.janelia.org/lab/rubin-lab/tools-reagents
Genetic reagent
(Drosophila melanogaster)
pJFRC28-10XUAS-
IVS-GFP-p10(attP40)
von Reyn et al., 2014
Genetic reagent
(Drosophila melanogaster)
pJFRC200-
10XUASIVS-
myr::smGFP-HA in
attP18
Aso et al., 2014
Genetic reagent
(Drosophila melanogaster)
pJFRC51-3xUASAso et al., 2014
Genetic reagent
(Drosophila melanogaster)
Syt::smGFP-HA
in su(Hw) attP1
Nern et al., 2015
Genetic reagent
(Drosophila melanogaster)
pJFRC225-5xUAS-IVS-
myr::smGFP-FLAG in
VK00005
Nern et al., 2015http://flybase.org/reports/FBrf0228639.html
Genetic reagent
(Drosophila melanogaster)
20XUAS-CsChrimson-
mVenus trafficked in
attP18
Klapoetke et al., 2014http://flybase.org/reports/FBrf0224686
Genetic reagent
(Drosophila melanogaster)
teashirt-GAL80Rubinstein et al., 2010
Antibodync82 supernatantMouse α-bruchpilot.Developmental Studies
Hybridoma Bank. # nc82-s
https://www.janelia.org/sites/default/files/Project%20Teams/Fly%20Light/FL%20Protocol%20-%20Adult%20IHC%20-%20Split%20Screen_1.pdf
Antibodyrabbit polyclonal
anti-GFP
Thermo Fisher ScientificCat #: A-11122;
RRID: AB_221569
AntibodyAlexa Fluor 488 goat
anti-rabbit
Thermo Fisher ScientificCat #: A-11034;
RRID: AB_2576217
AntibodyAlexa Fluor 568 goat
anti-mouse
Thermo Fisher ScientificCat #: A-11031;
RRID: AB_144696
Chemical compound, drugparaformaldehydeElectron Microscopy
Sciences
15713-Shttps://www.janelia.org/sites/default/files/Project%20Teams/Fly%20Light/FL%20Recipe%20-%20PFA_2.pdf
Chemical compound, drugTriton X-100Sigma AldrichX100https://www.janelia.org/sites/default/files/Project%20Teams/Fly%20Light/FL%20Protocol%20-%20Adult%20IHC%20-%20Split%20Screen_1.pdf
Chemical compound, drugDPX MountantElectron Microscopy
Sciences
#13512https://www.janelia.org/sites/default/files/Project%20Teams/Fly%20Light/FL%20Protocol%20-%20DPX%20Mounting_0.pdf
Chemical compound, drugxyleneFisher Scientificx5-500https://www.janelia.org/sites/default/files/Project%20Teams/Fly%20Light/FL%20Protocol%20-%20DPX%20Mounting_0.pdf

Additional files

Supplementary file 1

Morphological characteristics of identified descending neurons.

Column 1: DN cell type; column 2: maximum observed number of neurons in a cell type from a single preparation; column 3: maximum cell count for that type in a single sample; column 4: example Gen-1 GAL4 or split-GAL4 line with expression in the cell type; column 5: Y, yes, or N, no whether the cell type contains projections across the midline; column 6: indicates whether the axon traverses the cervical connective ipsilateral (I) or contralateral (C) to the cell body; columns under ‘Brain,’ ‘VNC,’ ‘Tract’: number indicates whether the cell type has neurites in the specified neuropil that are smooth (1), varicose (2), or both (3); 0 indicates the cell type has no innervation to the specified compartment; ‘Tract’ refers to the VNC tract traversed by the axon.

https://doi.org/10.7554/eLife.34272.054
Supplementary file 2

Descending neurons with similar morphology reported in Drosophila melanogaster and other species.

https://doi.org/10.7554/eLife.34272.055
Supplementary file 3

DN Split-GAL4 lines generated and used in this study.

‘Line name’ refers to the split-GAL4 stock number by which the line can be ordered. ‘Genotype stock name’ indicates which Gen1 parent lines were crossed to create the split line. ‘DN type’ indicates which DN cell type is targeted by the line. ‘Sample’ is the number of samples for which driver line expression was visualized (see Materials and methods). ‘Stochastic pattern’ is the number of samples that showed an expression patter deviant from the most common expression pattern observed. ‘bg’ indicates the approximate number of off-target cell types that also have expression using the driver line.

https://doi.org/10.7554/eLife.34272.056
Supplementary file 4

Interneuron and motor neuron split-GAL4 lines generated in this study.

https://doi.org/10.7554/eLife.34272.057
Supplementary file 5

More broadly expressing DN split-GAL4 lines made in this study.

These lines were not used in the present analysis. A rough quality score is provided for each line indicating it sparseness: Quality A, sparse lines without background expression; Quality B, lines with limited background expression or <5 off-target cell types; Quality C, lines with background expression.

https://doi.org/10.7554/eLife.34272.058
Supplementary file 6

Abbreviations used.

https://doi.org/10.7554/eLife.34272.059
Transparent reporting form
https://doi.org/10.7554/eLife.34272.060

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