TY - JOUR TI - Robust manipulation of the behavior of Drosophila melanogaster by a fungal pathogen in the laboratory AU - Elya, Carolyn AU - Lok, Tin Ching AU - Spencer, Quinn E AU - McCausland, Hayley AU - Martinez, Ciera C AU - Eisen, Michael A2 - Lazzaro, Brian P A2 - Wittkopp, Patricia J VL - 7 PY - 2018 DA - 2018/07/26 SP - e34414 C1 - eLife 2018;7:e34414 DO - 10.7554/eLife.34414 UR - https://doi.org/10.7554/eLife.34414 AB - Many microbes induce striking behavioral changes in their animal hosts, but how they achieve this is poorly understood, especially at the molecular level. Mechanistic understanding has been largely constrained by the lack of an experimental system amenable to molecular manipulation. We recently discovered a strain of the behavior-manipulating fungal pathogen Entomophthora muscae infecting wild Drosophila, and established methods to infect D. melanogaster in the lab. Lab-infected flies manifest the moribund behaviors characteristic of E. muscae infection: hours before death, they climb upward, extend their proboscides, affixing in place, then raise their wings, clearing a path for infectious spores to launch from their abdomens. We found that E. muscae invades the nervous system, suggesting a direct means by which the fungus could induce behavioral changes. Given the vast molecular toolkit available for D. melanogaster, we believe this new system will enable rapid progress in understanding how E. muscae manipulates host behavior. KW - behavior manipulation KW - entomopathogen KW - zombie flies KW - Entomophthora muscae JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -