TY - JOUR TI - RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation AU - Marshall, Richard S AU - Vierstra, Richard D A2 - Deshaies, Raymond J VL - 7 PY - 2018 DA - 2018/04/06 SP - e34532 C1 - eLife 2018;7:e34532 DO - 10.7554/eLife.34532 UR - https://doi.org/10.7554/eLife.34532 AB - 26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for granule assembly, not only influences the rate of proteasome degradation, but also impacts cell viability upon recovery from carbon starvation. PSG formation and concomitant protection against proteaphagy also occurs in Arabidopsis, suggesting that PSGs represent an evolutionarily conserved cache of proteasomes that can be rapidly re-mobilized based on energy availability. KW - autophagy KW - proteasome KW - proteaphagy KW - proteasome storage granules KW - starvation KW - ubiquitin JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -