TY - JOUR TI - An automated high-resolution in vivo screen in zebrafish to identify chemical regulators of myelination AU - Early, Jason J AU - Marshall-Phelps, Katy LH AU - Williamson, Jill M AU - Swire, Matthew AU - Kamadurai, Hari AU - Muskavitch, Marc AU - Lyons, David A A2 - Whitfield, Tanya T. VL - 7 PY - 2018 DA - 2018/07/06 SP - e35136 C1 - eLife 2018;7:e35136 DO - 10.7554/eLife.35136 UR - https://doi.org/10.7554/eLife.35136 AB - Myelinating oligodendrocytes are essential for central nervous system (CNS) formation and function. Their disruption is implicated in numerous neurodevelopmental, neuropsychiatric and neurodegenerative disorders. However, recent studies have indicated that oligodendrocytes may be tractable for treatment of disease. In recent years, zebrafish have become well established for the study of myelinating oligodendrocyte biology and drug discovery in vivo. Here, by automating the delivery of zebrafish larvae to a spinning disk confocal microscope, we were able to automate high-resolution imaging of myelinating oligodendrocytes in vivo. From there, we developed an image analysis pipeline that facilitated a screen of compounds with epigenetic and post-translational targets for their effects on regulating myelinating oligodendrocyte number. This screen identified novel compounds that strongly promote myelinating oligodendrocyte formation in vivo. Our imaging platform and analysis pipeline is flexible and can be employed for high-resolution imaging-based screens of broad interest using zebrafish. KW - automated chemical screening KW - myelination KW - VAST KW - zebrafish KW - oligodendrocyte JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -