TY - JOUR TI - A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions AU - Eisler, Stephan A AU - Curado, Filipa AU - Link, Gisela AU - Schulz, Sarah AU - Noack, Melanie AU - Steinke, Maren AU - Olayioye, Monilola A AU - Hausser, Angelika A2 - Pfeffer, Suzanne R A2 - Malhotra, Vivek VL - 7 PY - 2018 DA - 2018/07/20 SP - e35907 C1 - eLife 2018;7:e35907 DO - 10.7554/eLife.35907 UR - https://doi.org/10.7554/eLife.35907 AB - Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCĪµ that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions. KW - golgi complex KW - RhoGTPase KW - microtubules KW - vesicle formation JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -