The Aquilegia genome provides insight into adaptive radiation and reveals an extraordinarily polymorphic chromosome with a unique history
- Austrian Academy of Sciences, Austria
- University of California, Santa Barbara, United States
- Masaryk University, Czech Republic
- Department of Energy, United States
- Centre of the Region Haná for Biotechnological and Agricultural Research, Czech Republic
- Harvard University, United States
Abstract
The columbine genus Aquilegia is a classic example of an adaptive radiation, involving a wide variety of pollinators and habitats. Here we present the genome assembly of A. coerulea 'Goldsmith', complemented by high-coverage sequencing data from 10 wild species covering the world-wide distribution. Our analyses reveal extensive allele sharing among species, and demonstrate that introgression and selection played a role in the Aquilegia radiation. We also present the remarkable discovery that the evolutionary history of an entire chromosome differs from that of the rest of the genome - a phenomenon which we do not fully understand, but which highlights the need to consider chromosomes in an evolutionary context.
Data availability
Species resequencingA. barnebyi (SRR7965809), A. aurea (SRR405095), A. vulgaris (SRR404349), A. sibirica (SRR405090), A. formosa (SRR408554), A. japonica (SRR413499), A. oxysepala (SRR413921), A. longissima (SRR7965810), A. chrysantha (SRR408559), A. pubescens (SRR7943924) are available in the Short Read Archive(https://www.ncbi.nlm.nih.gov/sra).Whole genome Aquilegia coerulea 'Goldsmith'Sanger sequences used for genome assembly are available in the NCBI Trace Archive (https://www.ncbi.nlm.nih.gov/Traces).Aquilegia coerulea 'Goldsmith' ESTsAvailable in the NCBI Short Read Archive (SRR505574-SRR505578)Aquilegia formosa 412 ESTsAvailable in the NCBI dbEST (https://www.ncbi.nlm.nih.gov/dbEST/)Aquilegia coerulea 'Goldsmith' X Aquilegia chrysantha mapping populationAvailable in the NCBI Short Read Archive (SRR8000449-SRR8000976)Aquilegia formosa x Aquilegia pubescens mapping populationAvailable in the NCBI Short Read Archive (Bioproject PRJNA489508).grandparentspub.1 (SRR7943925), pub.2 (SRR7943924), form.1 (SRR7790646), form.2 (SRR408554)F1sF1.1 (SRR7943926), F1.2 (SRR7943927)F2sSRR7814612-SRR7814614, SRR7814616-SRR7814619, SRR7814622, SRR7814624-SRR7814686, SRR7826362- SRR7826624RNAseqAvailable in the NCBI Short Read Archive: see Supplementary Table 5 for more details.Other filesA vcf containing biallelic SNPs called in all ten Aquilegia species and Semiaquilegia (AQ.Semi.all.biallelic.SNPs.vcf.gz) and text files of genomic positions passing filtration (AQ.only.kept.positions.txt.gz and AQ.Semi.kept.positions.txt.gz) are available for download at Dryad (doi:10.5061/dryad.j4j12v0).URLsThe A. coerulea 'Goldsmith' v3.1 genome release is available at: https://phytozome.jgi.doe.gov/
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Data from: The Aquilegi a genome provides insight into adaptive radiation and reveals an extraordinarily polymorphic chromosome with a unique historyAvailable at Dryad Digital Repository under a CC0 Public Domain Dedication.
Article and author information
Author details
Funding
Czech Science Foundation (P501/12/G090)
- Martin A Lysak
CEITEC 2020 (LQ1601)
- Martin A Lysak
National Program of Sustainability I (LO1204)
- Miroslava Karafiátová
Austrian Science Funds (FWF DK W1225-B20)
- Gökçe Aköz
University of California, Santa Barbara (Harvey L. Karp Discovery Award)
- Evangeline S Ballerini
National Institutes of Health (Ruth L. Kirschstein National Research Service Award F32GM103154)
- Evangeline S Ballerini
National Science Foundation (IOS 1456317)
- Scott A Hodges
National Science Foundation (DEB 1311390)
- Nathan J Derieg
- Scott A Hodges
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Gil McVean, Oxford University, United Kingdom
Version history
- Received: March 6, 2018
- Accepted: September 17, 2018
- Accepted Manuscript published: October 16, 2018 (version 1)
- Version of Record published: November 26, 2018 (version 2)
Copyright
© 2018, Filiault et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The Aquilegia genome provides insight into adaptive radiation and reveals an extraordinarily polymorphic chromosome with a unique history
eLife 7:e36426.
https://doi.org/10.7554/eLife.36426
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