TY - JOUR TI - Active presynaptic ribosomes in the mammalian brain, and altered transmitter release after protein synthesis inhibition AU - Scarnati, Matthew S AU - Kataria, Rahul AU - Biswas, Mohana AU - Paradiso, Kenneth G A2 - Monteggia, Lisa M A2 - Westbrook, Gary L VL - 7 PY - 2018 DA - 2018/10/30 SP - e36697 C1 - eLife 2018;7:e36697 DO - 10.7554/eLife.36697 UR - https://doi.org/10.7554/eLife.36697 AB - Presynaptic neuronal activity requires the localization of thousands of proteins that are typically synthesized in the soma and transported to nerve terminals. Local translation for some dendritic proteins occurs, but local translation in mammalian presynaptic nerve terminals is difficult to demonstrate. Here, we show an essential ribosomal component, 5.8S rRNA, at a glutamatergic nerve terminal in the mammalian brain. We also show active translation in nerve terminals, in situ, in brain slices demonstrating ongoing presynaptic protein synthesis in the mammalian brain. Shortly after inhibiting translation, the presynaptic terminal exhibits increased spontaneous release, an increased paired pulse ratio, an increased vesicle replenishment rate during stimulation trains, and a reduced initial probability of release. The rise and decay rates of postsynaptic responses were not affected. We conclude that ongoing protein synthesis can limit excessive vesicle release which reduces the vesicle replenishment rate, thus conserving the energy required for maintaining synaptic transmission. KW - local translation KW - presynaptic KW - synaptic terminals KW - synaptic transmission KW - efficiency KW - efficacy JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -