TY - JOUR TI - Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex AU - Seeman, Stephanie C AU - Campagnola, Luke AU - Davoudian, Pasha A AU - Hoggarth, Alex AU - Hage, Travis A AU - Bosma-Moody, Alice AU - Baker, Christopher A AU - Lee, Jung Hoon AU - Mihalas, Stefan AU - Teeter, Corinne AU - Ko, Andrew L AU - Ojemann, Jeffrey G AU - Gwinn, Ryder P AU - Silbergeld, Daniel L AU - Cobbs, Charles AU - Phillips, John AU - Lein, Ed AU - Murphy, Gabe AU - Koch, Christof AU - Zeng, Hongkui AU - Jarsky, Tim A2 - Slutsky, Inna A2 - Marder, Eve A2 - Sjöström, Per Jesper VL - 7 PY - 2018 DA - 2018/09/26 SP - e37349 C1 - eLife 2018;7:e37349 DO - 10.7554/eLife.37349 UR - https://doi.org/10.7554/eLife.37349 AB - Generating a comprehensive description of cortical networks requires a large-scale, systematic approach. To that end, we have begun a pipeline project using multipatch electrophysiology, supplemented with two-photon optogenetics, to characterize connectivity and synaptic signaling between classes of neurons in adult mouse primary visual cortex (V1) and human cortex. We focus on producing results detailed enough for the generation of computational models and enabling comparison with future studies. Here, we report our examination of intralaminar connectivity within each of several classes of excitatory neurons. We find that connections are sparse but present among all excitatory cell classes and layers we sampled, and that most mouse synapses exhibited short-term depression with similar dynamics. Synaptic signaling between a subset of layer 2/3 neurons, however, exhibited facilitation. These results contribute to a body of evidence describing recurrent excitatory connectivity as a conserved feature of cortical microcircuits. KW - electrophysiology KW - short term plasticity KW - cortical wiring JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -