TY - JOUR TI - Temporal profiling of redox-dependent heterogeneity in single cells AU - Radzinski, Meytal AU - Fassler, Rosi AU - Yogev, Ohad AU - Breuer, William AU - Shai, Nadav AU - Gutin, Jenia AU - Ilyas, Sidra AU - Geffen, Yifat AU - Tsytkin-Kirschenzweig, Sabina AU - Nahmias, Yaakov AU - Ravid, Tommer AU - Friedman, Nir AU - Schuldiner, Maya AU - Reichmann, Dana A2 - Chacinska, Agnieszka VL - 7 PY - 2018 DA - 2018/06/05 SP - e37623 C1 - eLife 2018;7:e37623 DO - 10.7554/eLife.37623 UR - https://doi.org/10.7554/eLife.37623 AB - Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe Grx1-roGFP2. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach, we defined a redox-dependent heterogeneity of yeast cells and characterized growth, as well as proteomic and transcriptomic profiles of distinctive redox subpopulations. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis. KW - redox sensors KW - redox biology KW - yeast KW - roGFP JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -