TY - JOUR TI - PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading AU - Rona, Gergely AU - Roberti, Domenico AU - Yin, Yandong AU - Pagan, Julia K AU - Homer, Harrison AU - Sassani, Elizabeth AU - Zeke, Andras AU - Busino, Luca AU - Rothenberg, Eli AU - Pagano, Michele A2 - Aguilera, Andrés VL - 7 PY - 2018 DA - 2018/07/09 SP - e38771 C1 - eLife 2018;7:e38771 DO - 10.7554/eLife.38771 UR - https://doi.org/10.7554/eLife.38771 AB - The mammalian FBXL10-RNF68-RNF2 ubiquitin ligase complex (FRRUC) mono-ubiquitylates H2A at Lys119 to repress transcription in unstressed cells. We found that the FRRUC is rapidly and transiently recruited to sites of DNA damage in a PARP1- and TIMELESS-dependent manner to promote mono-ubiquitylation of H2A at Lys119, a local decrease of H2A levels, and an increase of H2A.Z incorporation. Both the FRRUC and H2A.Z promote transcriptional repression, double strand break signaling, and homologous recombination repair (HRR). All these events require both the presence and activity of the FRRUC. Moreover, the FRRUC and its activity are required for the proper recruitment of BMI1-RNF2 and MEL18-RNF2, two other ubiquitin ligases that mono-ubiquitylate Lys119 in H2A upon genotoxic stress. Notably, whereas H2A.Z is not required for H2A mono-ubiquitylation, impairment of the latter results in the inhibition of H2A.Z incorporation. We propose that the recruitment of the FRRUC represents an early and critical regulatory step in HRR. KW - DNA damage response KW - DNA repair KW - FBXL10 KW - RNF68 KW - RNF2 KW - BMI1 KW - MEL18 KW - H2A.Z KW - PARP KW - TIMELESS JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -