TY - JOUR TI - Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner AU - Gupta, Neha AU - Lorsch, Jon R AU - Hinnebusch, Alan G A2 - Sonenberg, Nahum A2 - Manley, James L VL - 7 PY - 2018 DA - 2018/10/03 SP - e38892 C1 - eLife 2018;7:e38892 DO - 10.7554/eLife.38892 UR - https://doi.org/10.7554/eLife.38892 AB - DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC) formation at the initiation codon. We reconstituted Ded1 acceleration of 48S PIC assembly on native mRNAs in a pure system, and recapitulated increased Ded1-dependence of mRNAs that are Ded1-hyperdependent in vivo. Stem-loop (SL) structures in 5'-UTRs of native and synthetic mRNAs increased the Ded1 requirement to overcome their intrinsically low rates of 48S PIC recruitment. Ded1 acceleration of 48S assembly was greater in the presence of eIF4F, and domains mediating one or more Ded1 interactions with eIF4G or helicase eIF4A were required for efficient recruitment of all mRNAs; however, the relative importance of particular Ded1 and eIF4G domains were distinct for each mRNA. Our results account for the Ded1 hyper-dependence of mRNAs with structure-prone 5'-UTRs, and implicate an eIF4E·eIF4G·eIF4A·Ded1 complex in accelerating 48S PIC assembly on native mRNAs. KW - translation KW - initiation KW - Ded1 KW - eIF4G KW - yeast JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -