TY - JOUR TI - Comparisons between the ON- and OFF-edge motion pathways in the Drosophila brain AU - Shinomiya, Kazunori AU - Huang, Gary AU - Lu, Zhiyuan AU - Parag, Toufiq AU - Xu, C Shan AU - Aniceto, Roxanne AU - Ansari, Namra AU - Cheatham, Natasha AU - Lauchie, Shirley AU - Neace, Erika AU - Ogundeyi, Omotara AU - Ordish, Christopher AU - Peel, David AU - Shinomiya, Aya AU - Smith, Claire AU - Takemura, Satoko AU - Talebi, Iris AU - Rivlin, Patricia K AU - Nern, Aljoscha AU - Scheffer, Louis K AU - Plaza, Stephen M AU - Meinertzhagen, Ian A A2 - Borst, Alexander A2 - VijayRaghavan, K VL - 8 PY - 2019 DA - 2019/01/09 SP - e40025 C1 - eLife 2019;8:e40025 DO - 10.7554/eLife.40025 UR - https://doi.org/10.7554/eLife.40025 AB - Understanding the circuit mechanisms behind motion detection is a long-standing question in visual neuroscience. In Drosophila melanogaster, recently discovered synapse-level connectomes in the optic lobe, particularly in ON-pathway (T4) receptive-field circuits, in concert with physiological studies, suggest a motion model that is increasingly intricate when compared with the ubiquitous Hassenstein-Reichardt model. By contrast, our knowledge of OFF-pathway (T5) has been incomplete. Here, we present a conclusive and comprehensive connectome that, for the first time, integrates detailed connectivity information for inputs to both the T4 and T5 pathways in a single EM dataset covering the entire optic lobe. With novel reconstruction methods using automated synapse prediction suited to such a large connectome, we successfully corroborate previous findings in the T4 pathway and comprehensively identify inputs and receptive fields for T5. Although the two pathways are probably evolutionarily linked and exhibit many similarities, we uncover interesting differences and interactions that may underlie their distinct functional properties. KW - visual system KW - motion detection KW - electron microscopy KW - connectome KW - neuroanatomy KW - optic lobe JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -