TY - JOUR TI - Tuning of delta-protocadherin adhesion through combinatorial diversity AU - Bisogni, Adam J AU - Ghazanfar, Shila AU - Williams, Eric O AU - Marsh, Heather M AU - Yang, Jean YH AU - Lin, David M A2 - Stainier, Didier Y A2 - Maniatis, Tom VL - 7 PY - 2018 DA - 2018/12/14 SP - e41050 C1 - eLife 2018;7:e41050 DO - 10.7554/eLife.41050 UR - https://doi.org/10.7554/eLife.41050 AB - The delta-protocadherins (δ-Pcdhs) play key roles in neural development, and expression studies suggest they are expressed in combination within neurons. The extent of this combinatorial diversity, and how these combinations influence cell adhesion, is poorly understood. We show that individual mouse olfactory sensory neurons express 0–7 δ-Pcdhs. Despite this apparent combinatorial complexity, K562 cell aggregation assays revealed simple principles that mediate tuning of δ-Pcdh adhesion. Cells can vary the number of δ-Pcdhs expressed, the level of surface expression, and which δ-Pcdhs are expressed, as different members possess distinct apparent adhesive affinities. These principles contrast with those identified previously for the clustered protocadherins (cPcdhs), where the particular combination of cPcdhs expressed does not appear to be a critical factor. Despite these differences, we show δ-Pcdhs can modify cPcdh adhesion. Our studies show how intra- and interfamily interactions can greatly amplify the impact of this small subfamily on neuronal function. KW - delta-protocadherins KW - single cell KW - combinatorial KW - cell adhesion KW - olfactory KW - aggregation JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -