TY - JOUR TI - A combination of transcription factors mediates inducible interchromosomal contacts AU - Kim, Seungsoo AU - Dunham, Maitreya J AU - Shendure, Jay A2 - Lee, Jeannie T A2 - Struhl, Kevin A2 - Fraser, Peter J A2 - Chu, Hsueh ping VL - 8 PY - 2019 DA - 2019/05/13 SP - e42499 C1 - eLife 2019;8:e42499 DO - 10.7554/eLife.42499 UR - https://doi.org/10.7554/eLife.42499 AB - The genome forms specific three-dimensional contacts in response to cellular or environmental conditions. However, it remains largely unknown which proteins specify and mediate such contacts. Here we describe an assay, MAP-C (Mutation Analysis in Pools by Chromosome conformation capture), that simultaneously characterizes the effects of hundreds of cis or trans-acting mutations on a chromosomal contact. Using MAP-C, we show that inducible interchromosomal pairing between HAS1pr-TDA1pr alleles in saturated cultures of Saccharomyces yeast is mediated by three transcription factors, Leu3, Sdd4 (Ypr022c), and Rgt1. The coincident, combined binding of all three factors is strongest at the HAS1pr-TDA1pr locus and is also specific to saturated conditions. We applied MAP-C to further explore the biochemical mechanism of these contacts, and find they require the structured regulatory domain of Rgt1, but no known interaction partners of Rgt1. Altogether, our results demonstrate MAP-C as a powerful method for dissecting the mechanistic basis of chromosome conformation. KW - saturation mutagenesis KW - mutational scanning KW - chromosome conformation KW - 3C KW - homolog pairing KW - transcription factors JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -