TY - JOUR TI - Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping AU - Tabor, Kathryn M AU - Marquart, Gregory D AU - Hurt, Christopher AU - Smith, Trevor S AU - Geoca, Alexandra K AU - Bhandiwad, Ashwin A AU - Subedi, Abhignya AU - Sinclair, Jennifer L AU - Rose, Hannah M AU - Polys, Nicholas F AU - Burgess, Harold A A2 - Raman, Indira M A2 - Stainier, Didier Y A2 - Wyart, Claire VL - 8 PY - 2019 DA - 2019/02/08 SP - e42687 C1 - eLife 2019;8:e42687 DO - 10.7554/eLife.42687 UR - https://doi.org/10.7554/eLife.42687 AB - Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies. KW - Cre KW - Gal4 KW - intersectional genetics KW - brain atlas KW - registration KW - imaging JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -