TY - JOUR TI - Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury AU - Farbehi, Nona AU - Patrick, Ralph AU - Dorison, Aude AU - Xaymardan, Munira AU - Janbandhu, Vaibhao AU - Wystub-Lis, Katharina AU - Ho, Joshua WK AU - Nordon, Robert E AU - Harvey, Richard P A2 - Morrisey, Edward A2 - Dietz, Harry C VL - 8 PY - 2019 DA - 2019/03/26 SP - e43882 C1 - eLife 2019;8:e43882 DO - 10.7554/eLife.43882 UR - https://doi.org/10.7554/eLife.43882 AB - Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration. KW - heart KW - myocardial infarction KW - scRNA-seq JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -