TY - JOUR TI - Anion channel SLAH3 is a regulatory target of chitin receptor-associated kinase PBL27 in microbial stomatal closure AU - Liu, Yi AU - Maierhofer, Tobias AU - Rybak, Katarzyna AU - Sklenar, Jan AU - Breakspear, Andy AU - Johnston, Matthew G AU - Fliegmann, Judith AU - Huang, Shouguang AU - Roelfsema, M Rob G AU - Felix, Georg AU - Faulkner, Christine AU - Menke, Frank LH AU - Geiger, Dietmar AU - Hedrich, Rainer AU - Robatzek, Silke A2 - Zhou, Jian-Min A2 - Hardtke, Christian S A2 - Hoth, Stefan VL - 8 PY - 2019 DA - 2019/09/16 SP - e44474 C1 - eLife 2019;8:e44474 DO - 10.7554/eLife.44474 UR - https://doi.org/10.7554/eLife.44474 AB - In plants, antimicrobial immune responses involve the cellular release of anions and are responsible for the closure of stomatal pores. Detection of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) induces currents mediated via slow-type (S-type) anion channels by a yet not understood mechanism. Here, we show that stomatal closure to fungal chitin is conferred by the major PRRs for chitin recognition, LYK5 and CERK1, the receptor-like cytoplasmic kinase PBL27, and the SLAH3 anion channel. PBL27 has the capacity to phosphorylate SLAH3, of which S127 and S189 are required to activate SLAH3. Full activation of the channel entails CERK1, depending on PBL27. Importantly, both S127 and S189 residues of SLAH3 are required for chitin-induced stomatal closure and anti-fungal immunity at the whole leaf level. Our results demonstrate a short signal transduction module from MAMP recognition to anion channel activation, and independent of ABA-induced SLAH3 activation. KW - MAMP KW - PAMP-triggered immunity KW - PTI KW - CERK1 KW - LYK5 KW - SLAH3 JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -