TY - JOUR TI - Microtubule plus-end dynamics link wound repair to the innate immune response AU - Taffoni, Clara AU - Omi, Shizue AU - Huber, Caroline AU - Mailfert, Sébastien AU - Fallet, Mathieu AU - Rupprecht, Jean-François AU - Ewbank, Jonathan J AU - Pujol, Nathalie A2 - Lechler, Terry A2 - Stainier, Didier YR A2 - Bement, William M A2 - Chisholm, Andrew D VL - 9 PY - 2020 DA - 2020/01/29 SP - e45047 C1 - eLife 2020;9:e45047 DO - 10.7554/eLife.45047 UR - https://doi.org/10.7554/eLife.45047 AB - The skin protects animals from infection and physical damage. In Caenorhabditis elegans, wounding the epidermis triggers an immune reaction and a repair response, but it is not clear how these are coordinated. Previous work implicated the microtubule cytoskeleton in the maintenance of epidermal integrity (Chuang et al., 2016). Here, by establishing a simple wounding system, we show that wounding provokes a reorganisation of plasma membrane subdomains. This is followed by recruitment of the microtubule plus end-binding protein EB1/EBP-2 around the wound and actin ring formation, dependent on ARP2/3 branched actin polymerisation. We show that microtubule dynamics are required for the recruitment and closure of the actin ring, and for the trafficking of the key signalling protein SLC6/SNF-12 toward the injury site. Without SNF-12 recruitment, there is an abrogation of the immune response. Our results suggest that microtubule dynamics coordinate the cytoskeletal changes required for wound repair and the concomitant activation of innate immunity. KW - wound healing KW - innate innmunity KW - microtubules KW - actin KW - EB1 KW - signaling JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -