TY - JOUR TI - The long non-coding RNA Cerox1 is a post transcriptional regulator of mitochondrial complex I catalytic activity AU - Sirey, Tamara M AU - Roberts, Kenny AU - Haerty, Wilfried AU - Bedoya-Reina, Oscar AU - Rogatti-Granados, Sebastian AU - Tan, Jennifer Y AU - Li, Nick AU - Heather, Lisa C AU - Carter, Roderick N AU - Cooper, Sarah AU - Finch, Andrew J AU - Wills, Jimi AU - Morton, Nicholas M AU - Marques, Ana Claudia AU - Ponting, Chris P A2 - Weigel, Detlef VL - 8 PY - 2019 DA - 2019/05/02 SP - e45051 C1 - eLife 2019;8:e45051 DO - 10.7554/eLife.45051 UR - https://doi.org/10.7554/eLife.45051 AB - To generate energy efficiently, the cell is uniquely challenged to co-ordinate the abundance of electron transport chain protein subunits expressed from both nuclear and mitochondrial genomes. How an effective stoichiometry of this many constituent subunits is co-ordinated post-transcriptionally remains poorly understood. Here we show that Cerox1, an unusually abundant cytoplasmic long noncoding RNA (lncRNA), modulates the levels of mitochondrial complex I subunit transcripts in a manner that requires binding to microRNA-488-3p. Increased abundance of Cerox1 cooperatively elevates complex I subunit protein abundance and enzymatic activity, decreases reactive oxygen species production, and protects against the complex I inhibitor rotenone. Cerox1 function is conserved across placental mammals: human and mouse orthologues effectively modulate complex I enzymatic activity in mouse and human cells, respectively. Cerox1 is the first lncRNA demonstrated, to our knowledge, to regulate mitochondrial oxidative phosphorylation and, with miR-488-3p, represent novel targets for the modulation of complex I activity. KW - mitochondria KW - energy metabolism KW - long noncoding RNA KW - miRNA KW - post-transcriptional regulation JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -