TY - JOUR TI - Single-cell lineage tracing by endogenous mutations enriched in transposase accessible mitochondrial DNA AU - Xu, Jin AU - Nuno, Kevin AU - Litzenburger, Ulrike M AU - Qi, Yanyan AU - Corces, M Ryan AU - Majeti, Ravindra AU - Chang, Howard Y A2 - Levine, Ross L A2 - Bronner, Marianne E A2 - Levine, Ross L VL - 8 PY - 2019 DA - 2019/04/08 SP - e45105 C1 - eLife 2019;8:e45105 DO - 10.7554/eLife.45105 UR - https://doi.org/10.7554/eLife.45105 AB - Simultaneous measurement of cell lineage and cell fates is a longstanding goal in biomedicine. Here we describe EMBLEM, a strategy to track cell lineage using endogenous mitochondrial DNA variants in ATAC-seq data. We show that somatic mutations in mitochondrial DNA can reconstruct cell lineage relationships at single cell resolution with high sensitivity and specificity. Using EMBLEM, we define the genetic and epigenomic clonal evolution of hematopoietic stem cells and their progenies in patients with acute myeloid leukemia. EMBLEM extends lineage tracing to any eukaryotic organism without genetic engineering. KW - ATAC-seq KW - mitochondrial DNA mutations KW - lineage tracing KW - hematopoietic stem cells KW - acute myeloid leukemia KW - EMBLEM JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -