TY - JOUR TI - Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation AU - Strutt, Helen AU - Gamage, Jessica AU - Strutt, David A2 - Nathans, Jeremy A2 - Banerjee, Utpal VL - 8 PY - 2019 DA - 2019/05/15 SP - e45107 C1 - eLife 2019;8:e45107 DO - 10.7554/eLife.45107 UR - https://doi.org/10.7554/eLife.45107 AB - The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of neighbouring cells. This asymmetric segregation is regulated by phosphorylation through poorly understood mechanisms. We show that loss of phosphorylation of the core protein Strabismus in the Drosophila pupal wing increases its stability and promotes its clustering at intercellular junctions, and that Prickle negatively regulates Strabismus phosphorylation. Additionally, loss of phosphorylation of Dishevelled – which normally localises to opposite cell edges to Strabismus – reduces its stability at junctions. Moreover, both phosphorylation events are independently mediated by Casein Kinase Iε. We conclude that Casein Kinase Iε phosphorylation acts as a switch, promoting Strabismus mobility and Dishevelled immobility, thus enhancing sorting of these proteins to opposite cell edges. KW - planar cell polarity KW - PCP KW - Strabismus KW - Casein Kinase Iε KW - phosphorylation KW - Dishevelled JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -