TY - JOUR TI - Natural selection and repeated patterns of molecular evolution following allopatric divergence AU - Dong, Yibo AU - Chen, Shichao AU - Cheng, Shifeng AU - Zhou, Wenbin AU - Ma, Qing AU - Chen, Zhiduan AU - Fu, Cheng-Xin AU - Liu, Xin AU - Zhao, Yun-peng AU - Soltis, Pamela S AU - Wong, Gane Ka-Shu AU - Soltis, Douglas E AU - Xiang, Qiu-Yun(Jenny) A2 - Kliebenstein, Daniel J A2 - Wittkopp, Patricia J A2 - Savolainen, Vincent VL - 8 PY - 2019 DA - 2019/08/02 SP - e45199 C1 - eLife 2019;8:e45199 DO - 10.7554/eLife.45199 UR - https://doi.org/10.7554/eLife.45199 AB - Although geographic isolation is a leading driver of speciation, the tempo and pattern of divergence at the genomic level remain unclear. We examine genome-wide divergence of putatively single-copy orthologous genes (POGs) in 20 allopatric species/variety pairs from diverse angiosperm clades, with 16 pairs reflecting the classic eastern Asia-eastern North America floristic disjunction. In each pair, >90% of POGs are under purifying selection, and <10% are under positive selection. A set of POGs are under strong positive selection, 14 of which are shared by 10–15 pairs, and one shared by all pairs; 15 POGs are annotated to biological processes responding to various stimuli. The relative abundance of POGs under different selective forces exhibits a repeated pattern among pairs despite an ~10 million-year difference in divergence time. Species divergence times are positively correlated with abundance of POGs under moderate purifying selection, but negatively correlated with abundance of POGs under strong purifying selection. KW - genetic divergence KW - allopatric/geographic speciation KW - transcriptome sequencing KW - eastern Asia-eastern North America floristic disjunction KW - molecular evolution KW - positive selection JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -