MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy
- University of Zurich, Switzerland
Abstract
The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.
Data availability
Datasets 1 and 2 are publicly available for online visualization and download at https://neurodata.io/data/templier2019. Code is at https://github.com/templiert/MagC.
Article and author information
Author details
Funding
ETH Zurich Foundation ETH Grant (42 15-1)
- Thomas Templier
Innosuisse-Swiss National Foundation Bridge Proof of Concept (173825)
- Thomas Templier
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal experiments were approved by the Veterinary office of Canton Zurich (207/2013).
Copyright
© 2019, Templier
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy
eLife 8:e45696.
https://doi.org/10.7554/eLife.45696
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