TY - JOUR TI - Notch signaling restricts FGF pathway activation in parapineal cells to promote their collective migration AU - Wei, Lu AU - Al Oustah, Amir AU - Blader, Patrick AU - Roussigné, Myriam A2 - Bronner, Marianne E A2 - Chitnis, Ajay B VL - 8 PY - 2019 DA - 2019/09/09 SP - e46275 C1 - eLife 2019;8:e46275 DO - 10.7554/eLife.46275 UR - https://doi.org/10.7554/eLife.46275 AB - Coordinated migration of cell collectives is important during embryonic development and relies on cells integrating multiple mechanical and chemical cues. Recently, we described that focal activation of the FGF pathway promotes the migration of the parapineal in the zebrafish epithalamus. How FGF activity is restricted to leading cells in this system is, however, unclear. Here, we address the role of Notch signaling in modulating FGF activity within the parapineal. While Notch loss-of-function results in an increased number of parapineal cells activating the FGF pathway, global activation of Notch signaling decreases it; both contexts result in defects in parapineal migration and specification. Decreasing or increasing FGF signaling in a Notch loss-of-function context respectively rescues or aggravates parapineal migration defects without affecting parapineal cells specification. We propose that Notch signaling controls the migration of the parapineal through its capacity to restrict FGF pathway activation to a few leading cells. KW - collective cell migration KW - notch signaling KW - FGF pathway KW - left right asymmetry JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -