TY - JOUR TI - The structure of the yeast Ctf3 complex AU - Hinshaw, Stephen M AU - Dates, Andrew N AU - Harrison, Stephen C A2 - Musacchio, Andrea A2 - Wolberger, Cynthia A2 - Musacchio, Andrea A2 - Yu, Hongtao VL - 8 PY - 2019 DA - 2019/06/13 SP - e48215 C1 - eLife 2019;8:e48215 DO - 10.7554/eLife.48215 UR - https://doi.org/10.7554/eLife.48215 AB - Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function. KW - Mitosis KW - Kinetochore KW - Cryo-EM JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -