TY - JOUR TI - A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells AU - Abu-Shah, Enas AU - Demetriou, Philippos AU - Bálint, Štefan AU - Mayya, Viveka AU - Kutuzov, Mikhail A AU - Dushek, Omer AU - Dustin, Michael L A2 - Sakaguchi, Shimon A2 - Rath, Satyajit VL - 8 PY - 2019 DA - 2019/09/25 SP - e48221 C1 - eLife 2019;8:e48221 DO - 10.7554/eLife.48221 UR - https://doi.org/10.7554/eLife.48221 AB - Research in the field of human immunology is restricted by the lack of a system that reconstitutes the in-situactivation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single-cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation. As a proof of concept, we recapitulate immunological phenomenon such as CD4 T-cells' help to CD8 T-cells through enhanced maturation of DCs and the effect of PD-1 checkpoint blockades. In addition, we characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity. KW - T-cells KW - antigen presenting cells KW - 3D model system KW - TCR engineering KW - immunotherapy JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -