Regulated spindle orientation buffers tissue growth in the epidermis

The epidermis is a proliferative tissue that turns over repeatedly throughout life. Remarkably, in aged sun-exposed skin, homeostasis and the architecture of the epidermis is maintained despite prevalent clones of cells containing oncogenic mutations (Martincorena et al., 2015). Recent work has highlighted oncogene-induced differentiation and active elimination of mutant clones as two mechanisms of epidermal robustness, suggesting there are multiple modes of response to perturbation (Brown et al., 2017; Ying et al., 2018). Homeostasis requires that gain of cells (proliferation) must be balanced with loss of cells (differentiation or cell death). One possible mechanism to control homeostasis is regulated spindle orientation and asymmetric cell divisions. These divisions do not increase progenitor number, but rather commit one daughter to differentiation. Embryonic epidermal progenitors can divide parallel to the basement membrane, resulting in two basal daughter cells, which we term symmetric divisions. These cells can also divide perpendicular to the basement membrane (Lechler and Fuchs, 2005; Poulson and Lechler, 2010; Smart, 1970; Williams et al., 2011). Perpendicular divisions are termed asymmetric divisions because they contribute one daughter cell to the differentiated suprabasal cell layer and do not alter the number of basal progenitor cells. Because of these properties, regulated spindle orientation is a candidate for providing resilience to homeostatic perturbation. In the embryonic epidermis, asymmetric divisions are used to drive stratification of the skin (Lechler and Fuchs, 2005; Smart, 1970; Williams et al., 2011). However, in adult backskin most divisions are planar (symmetric) and the utility of regulated spindle orientation has not been explored (Ipponjima et al., 2016; Rompolas et al., 2016). Here, we examine the possibility that regulated spindle orientation can buffer the effects of perturbed homeostasis.

We began by examining whether spindle orientation was altered when developmental or adult homeostatic proliferation was experimentally altered. In the embryonic mouse epidermis, where proliferation is high, the ratio of perpendicular to planar divisions is approximately 70:30 (Lechler and Fuchs, 2005; Smart, 1970). To inhibit basal cell cycle progression, we used a basal epidermal keratin 14 promoter to drive a doxycycline-inducible allele of Cdkn1b, a CDK1 inhibitor (K14-rtTA;tetO-CDKN1b) (Pruitt et al., 2013). Quantitation of the percentage of cells expressing the mitotic marker phospho-histone H3 (pHH3) revealed a significant decrease in basal cell proliferation (Figure 1A). This also resulted in a significant reduction in perpendicular divisions, from 70% to 35%, with a concomitant increase in planar divisions (from 22% to 42%) (Figure 1B,C). We measured spindle angles as shown in Figure 1—figure supplement 1. This data is consistent with the need for increased rates of planar divisions to maintain the surface area of the epidermis in a rapidly growing embryo.

Figure 1 with 3 supplements see all

Download asset Open asset

In contrast to the embryo, the proliferation rate in the adult backskin is low. Live imaging has shown that most divisions occur symmetrically and that basal cells delaminate from the basement membrane to populate suprabasal cell layers (Ipponjima et al., 2016; Rompolas et al., 2016). Consistent with this observation, we see that the majority of mitotic spindles are planar in adult backskin (Figure 1E). Notably, there is regional variability in epidermis from different anatomical areas in both proliferation and spindle orientation. The epidermis of the footpad, which is thicker, has both a higher proliferation index and increased numbers of perpendicular divisions (Figure 1—figure supplement 2). Similar to the embryonic epidermis, expression of Cdkn1b caused a shift to more planar cell divisions in the paw epidermis (Figure 1—figure supplement 2E).

To induce hyperproliferation of adult backskin epidermis we topically applied the mitogen TPA. This resulted in thickening of the epidermis and a 6-fold increase in proliferation rate as measured by BrdU incorporation (Figure 1D). Notably, this treatment increased the percentage of perpendicular divisions from 20% to 40% (Figure 1E,F). Therefore, one of the responses to TPA-induced hyperproliferation in the epidermis is an increase in the rate of divisions that give rise to differentiated progeny, as seen by staining for the differentiation marker, keratin 10 (Figure 1—figure supplement 3). Together these data demonstrate correlations between proliferation and spindle orientation, and support the hypothesis that cells tune their division orientation in response to the needs of the surrounding tissue as a mechanism to promote robust homeostasis.

To directly determine whether regulated spindle orientation was important for epidermal homeostasis we examined the effects of mutating the nuclear mitotic apparatus protein, NuMA, which is essential for spindle orientation in embryonic skin (Williams et al., 2011; Seldin et al., 2016). We did not see significant phenotypes when we disrupted NuMA’s spindle orientation function in adult epidermis for short times (data not shown), consistent with findings that adult backskin homeostasis is normally maintained by delamination and differentiation rather than by regulated spindle orientation (Rompolas et al., 2016).

Given that tissues responded to TPA-induced hyperproliferation by increasing perpendicular divisions, we next asked whether this also occurred in response to an oncogenic perturbation. Mutations that activate Ras family members can drive squamous cell carcinoma and a significant percentage of squamous tumors carry Ras mutations (Daya-Grosjean et al., 1993; Pierceall et al., 1991; South et al., 2014). Unexpectedly, we found that expression of KRAS^G12D (at endogenous levels and only in the epidermis; K5CreER;KRAS^G12D/+) had distinct effects in backskin and footpad epidermis. While there was no significant change in division orientation ratios in the backskin, active K-Ras resulted in an increase in perpendicular divisions in the footpads (Figure 2A–D). These data rule out the possibility that active K-Ras inhibits regulated spindle orientation/asymmetric cell divisions as an oncogenic mechanism. It raised the alternative possibility that the increased perpendicular divisions may protect the tissue against overgrowth. We therefore addressed the molecular requirements for spindle orientation in skin expressing active K-Ras. We combined a NuMA mutation that disrupts embryonic spindle orientation with the KRAS^G12D oncogene (Seldin et al., 2016; Silk et al., 2009). This resulted in a significant shift of divisions from perpendicular to planar, consistent with NuMA being required for spindle orientation in this context (Figure 2E). On their own, mutations in NuMA resulted in a skewing of spindle orientations in the pawskin (Figure 2—figure supplement 1).

Figure 2 with 1 supplement see all

Download asset Open asset

The consequence of perturbing regulated spindle orientation in epidermis expressing active KRAS was dramatic. Within two weeks of recombination, large overgrowths appeared on the footpads and anogenital regions of the double mutant mice (K5Cre^ER;KRAS^G12D/+;NuMA^MTBDfl/fl) (Figure 3A). These features were not found in single KRAS^G12D or NuMA mutants, although KRAS^G12D alone was sufficient for formation of oral tumors similar to those that appeared in the double mutants. Most mice died within 3–4 weeks after recombination (Figure 3B).

Figure 3 with 1 supplement see all

Download asset Open asset

Histologic analysis of the double mutant paws demonstrated massive tissue overgrowth and papilloma formation (Figure 3D–G). Despite this dramatic change in tissue architecture, the basement membrane remained intact and differentiation was normal at early time points. Thus, this combination of mutations is not sufficient for invasion, at least in the short time period we were able to assay. The massive tissue overgrowth led to lethality, likely due, at least in part, to blockage of the oral cavity.

Similar to the loss of NuMA function in the adult epidermis, loss of spindle orientation alone is not detrimental in developing fly wings (Nakajima et al., 2013). However, tumors form when combined with additional mutations that inhibited apoptosis. Notably, we saw no genetic interaction when mutant p53 was combined with the NuMA^ΔMTBD deletion (Figure 3C). These data are consistent with the fact that apoptosis is not a major determinant of tissue homeostasis in the epidermis under normal conditions, and demonstrates a specificity for disrupted spindle organization working synergistically with only a subset of oncogenic drivers.

Histologic analysis revealed expansion of the stem cell pool in the KRAS^G12D; NuMA^ΔMTBD epidermis. To accommodate the increase in cell numbers, the basement membrane became highly involuted (Figure 3D–G). We quantitated this as basement membrane length per tissue length and found a 2–4 fold increase in this measure, corresponding to a 4–16 fold increase in area (Figure 3H). As cell density was not notably altered, this demonstrates an expansion of the basal stem cell pool. These data are consistent with regulated spindle orientation buffering the effects of oncogenic K-Ras. Similarly, we found that low-dose TPA treatment caused a subtle yet significant increase in basement membrane length in NuMA^ΔMTBD mice as compared to control mice (Figure 3—figure supplement 1).

Active K-Ras has both proliferative and anti-differentiative effects in different tissues. Because increased proliferation can drive increased asymmetric divisions (Figure 1), we asked whether the changes observed in K-Ras mutant skin could be due to oncogene-induced hyperproliferation. Notably, we found that constitutively active KRas expression is not sufficient to drive hyperproliferation in the paw skin, nor did it do so in combination with a mutation in NuMA (Figure 3I).

Previous work has suggested that active Ras can inhibit differentiation when overexpressed in the epidermis (Dajee et al., 2002). However, this has not been examined either in the adult pawskin or under endogenous levels of expression. In adults, cells commit to differentiation, detach from the basement membrane, and move upward. A small proportion of basal cells express the differentiation marker keratin 10 (K10) and can be fluorescently labeled in mice using established mouse lines (K10-rtTA;TRE-H2B-GFP) (Muroyama and Lechler, 2017; Figure 3J). In the pawskin, we found that ~10% of basal cells were labeled with H2B-GFP, and this fell to 0.4% two days after removal of doxycycline, consistent with these cells entering differentiation (n>200 cells). Additionally, H2B-GFP labeled-basal cells incorporated EdU at a lower rate than surrounding basal cells (28 ± 2.4% for K10^-ve basal cells verses 9 ± 3.0% for K10^+ve basal cells, n=3 mice, >400 cells/mouse). We quantitated differentiation by analyzing the percentage of basal cells that express keratin 10. Using the K10-rtTA;TRE-H2B-GFP genetic labeling system we found that differentiation was suppressed in KRAS^G12D expressing cells (Figure 3K). This was most dramatic in the paw epidermis, but was also observed in backskin to a lesser extent. While the mechanism underlying this suppression is not clear, we also found that the relative intensity of β4-integrin was increased in the mutant pawskin, in a similar manner to what was observed when K-Ras was overexpressed in the embryo (Dajee et al., 2002; Figure 3J). Robust adhesion and signaling through integrins is known to promote progenitor cell fate, and thus may contribute to the changes in differentiation that we observed.

Together, our data demonstrate that the epidermis can respond to changes in homeostasis by altering mitotic spindle orientation. Increasing perpendicular (asymmetric) divisions in response to either increased proliferation or decreased differentiation allows homeostasis to be reset without dramatically affecting tissue architecture. Our genetic analysis clearly shows that this is essential to prevent tissue overgrowth in an oncogenic background. In addition, we found that the epidermis responded differently to the same oncogenic insult at different anatomic sites. This likely reflects the differences in proliferation, differentiation, and rates of asymmetric cell division rates in different regions of the epidermis and suggests that distinct ‘second hits’ may be needed to collaborate with oncogenes in tissues with different mechanisms of achieving homeostasis.

Our data are consistent with the idea that both regulated spindle orientation and delamination/differentiation can be used to buffer basal cell number. Disruption of either one was insufficient to induce homeostatic defects due to compensation by the other. However, blockade of both homeostatic buffers resulted in tissue overgrowth and progenitor cell expansion. Notably, squamous cell carcinomas have been reported to have increased rates of perpendicular divisions as compared to normal epidermis (Beck et al., 2011; Siegle et al., 2014). It will be important to see whether regulated spindle orientation is protective against tumor development in these contexts.

There are no large datasets associated with this manuscript.

1. 1. Beck B
   2. Driessens G
   3. Goossens S
   4. Youssef KK
   5. Kuchnio A
   6. Caauwe A
   7. Sotiropoulou PA
   8. Loges S
   9. Lapouge G
   10. Candi A
   11. Mascre G
   12. Drogat B
   13. Dekoninck S
   14. Haigh JJ
   15. Carmeliet P
   16. Blanpain C

   (2011) A vascular niche and a VEGF–Nrp1 loop regulate the initiation and stemness of skin tumours

   Nature 478:399–403.

   https://doi.org/10.1038/nature10525

   • Google Scholar
2. 1. Brown S
   2. Pineda CM
   3. Xin T
   4. Boucher J
   5. Suozzi KC
   6. Park S
   7. Matte-Martone C
   8. Gonzalez DG
   9. Rytlewski J
   10. Beronja S
   11. Greco V

   (2017) Correction of aberrant growth preserves tissue homeostasis

   Nature 548:334–337.

   https://doi.org/10.1038/nature23304

   • PubMed
   • Google Scholar
3. 1. Dajee M
   2. Tarutani M
   3. Deng H
   4. Cai T
   5. Khavari PA

   (2002) Epidermal ras blockade demonstrates spatially localized ras promotion of proliferation and inhibition of differentiation

   Oncogene 21:1527–1538.

   https://doi.org/10.1038/sj.onc.1205287

   • PubMed
   • Google Scholar
4. 1. Daya-Grosjean L
   2. Robert C
   3. Drougard C
   4. Suarez H
   5. Sarasin A

   (1993)

   High mutation frequency in ras genes of skin tumors isolated from DNA repair deficient xeroderma pigmentosum patients

   Cancer Research 53:1625–1629.

   • PubMed
   • Google Scholar
5. 1. Ipponjima S
   2. Hibi T
   3. Nemoto T

   (2016) Three-Dimensional analysis of cell division orientation in epidermal basal layer using intravital Two-Photon microscopy

   PLOS ONE 11:e0163199.

   https://doi.org/10.1371/journal.pone.0163199

   • PubMed
   • Google Scholar
6. 1. Jackson EL
   2. Willis N
   3. Mercer K
   4. Bronson RT
   5. Crowley D
   6. Montoya R
   7. Jacks T
   8. Tuveson DA

   (2001) Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras

   Genes & Development 15:3243–3248.

   https://doi.org/10.1101/gad.943001

   • PubMed
   • Google Scholar
7. 1. Lechler T
   2. Fuchs E

   (2005) Asymmetric cell divisions promote stratification and differentiation of mammalian skin

   Nature 437:275–280.

   https://doi.org/10.1038/nature03922

   • PubMed
   • Google Scholar
8. 1. Marino S
   2. Vooijs M
   3. van Der Gulden H
   4. Jonkers J
   5. Berns A

   (2000)

   Induction of medulloblastomas in p53-null mutant mice by somatic inactivation of rb in the external granular layer cells of the cerebellum

   Genes & Development 14:994–1004.

   • PubMed
   • Google Scholar
9. 1. Martincorena I
   2. Roshan A
   3. Gerstung M
   4. Ellis P
   5. Van Loo P
   6. McLaren S
   7. Wedge DC
   8. Fullam A
   9. Alexandrov LB
   10. Tubio JM
   11. Stebbings L
   12. Menzies A
   13. Widaa S
   14. Stratton MR
   15. Jones PH
   16. Campbell PJ

   (2015) High burden and pervasive positive selection of somatic mutations in normal human skin

   Science 348:880–886.

   https://doi.org/10.1126/science.aaa6806

   • PubMed
   • Google Scholar
10. 1. Muroyama A
    2. Lechler T

    (2017) A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis

    eLife 6:e29834.

    https://doi.org/10.7554/eLife.29834

    • PubMed
    • Google Scholar
11. 1. Nakajima Y
    2. Meyer EJ
    3. Kroesen A
    4. McKinney SA
    5. Gibson MC

    (2013) Epithelial junctions maintain tissue architecture by directing planar spindle orientation

    Nature 500:359–362.

    https://doi.org/10.1038/nature12335

    • PubMed
    • Google Scholar
12. 1. Pierceall WE
    2. Goldberg LH
    3. Tainsky MA
    4. Mukhopadhyay T
    5. Ananthaswamy HN

    (1991) Ras gene mutation and amplification in human nonmelanoma skin cancers

    Molecular Carcinogenesis 4:196–202.

    https://doi.org/10.1002/mc.2940040306

    • PubMed
    • Google Scholar
13. 1. Poulson ND
    2. Lechler T

    (2010) Robust control of mitotic spindle orientation in the developing epidermis

    The Journal of Cell Biology 191:915–922.

    https://doi.org/10.1083/jcb.201008001

    • PubMed
    • Google Scholar
14. 1. Pruitt SC
    2. Freeland A
    3. Rusiniak ME
    4. Kunnev D
    5. Cady GK

    (2013) Cdkn1b overexpression in adult mice alters the balance between genome and tissue ageing

    Nature Communications 4:2626.

    https://doi.org/10.1038/ncomms3626

    • PubMed
    • Google Scholar
15. 1. Rompolas P
    2. Mesa KR
    3. Kawaguchi K
    4. Park S
    5. Gonzalez D
    6. Brown S
    7. Boucher J
    8. Klein AM
    9. Greco V

    (2016) Spatiotemporal coordination of stem cell commitment during epidermal homeostasis

    Science 352:1471–1474.

    https://doi.org/10.1126/science.aaf7012

    • PubMed
    • Google Scholar
16. 1. Seldin L
    2. Muroyama A
    3. Lechler T

    (2016) NuMA-microtubule interactions are critical for spindle orientation and the morphogenesis of diverse epidermal structures

    eLife 5:e12504.

    https://doi.org/10.7554/eLife.12504

    • PubMed
    • Google Scholar
17. 1. Siegle JM
    2. Basin A
    3. Sastre-Perona A
    4. Yonekubo Y
    5. Brown J
    6. Sennett R
    7. Rendl M
    8. Tsirigos A
    9. Carucci JA
    10. Schober M

    (2014) SOX2 is a cancer-specific regulator of tumour initiating potential in cutaneous squamous cell carcinoma

    Nature Communications 5:4511.

    https://doi.org/10.1038/ncomms5511

    • PubMed
    • Google Scholar
18. 1. Silk AD
    2. Holland AJ
    3. Cleveland DW

    (2009) Requirements for NuMA in maintenance and establishment of mammalian spindle poles

    The Journal of Cell Biology 184:677–690.

    https://doi.org/10.1083/jcb.200810091

    • PubMed
    • Google Scholar
19. 1. Smart IH

    (1970) Variation in the plane of cell cleavage during the process of stratification in the mouse epidermis

    British Journal of Dermatology 82:276–282.

    https://doi.org/10.1111/j.1365-2133.1970.tb12437.x

    • PubMed
    • Google Scholar
20. 1. South AP
    2. Purdie KJ
    3. Watt SA
    4. Haldenby S
    5. den Breems N
    6. Dimon M
    7. Arron ST
    8. Kluk MJ
    9. Aster JC
    10. McHugh A
    11. Xue DJ
    12. Dayal JH
    13. Robinson KS
    14. Rizvi SH
    15. Proby CM
    16. Harwood CA
    17. Leigh IM

    (2014) NOTCH1 mutations occur early during cutaneous squamous cell carcinogenesis

    Journal of Investigative Dermatology 134:2630–2638.

    https://doi.org/10.1038/jid.2014.154

    • PubMed
    • Google Scholar
21. 1. Williams SE
    2. Beronja S
    3. Pasolli HA
    4. Fuchs E

    (2011) Asymmetric cell divisions promote Notch-dependent epidermal differentiation

    Nature 470:353–358.

    https://doi.org/10.1038/nature09793

    • Google Scholar
22. 1. Ying Z
    2. Sandoval M
    3. Beronja S

    (2018) Oncogenic activation of PI3K induces progenitor cell differentiation to suppress epidermal growth

    Nature Cell Biology 20:1256–1266.

    https://doi.org/10.1038/s41556-018-0218-9

    • PubMed
    • Google Scholar

Author details

1. Angel Morrow

   Department of Dermatology, Duke University, Durham, United States

   Present address

   Novozymes, Durham, United Kingdom

   Contribution

   Data curation, Formal analysis, Investigation, Methodology, Writing—review and editing

   Contributed equally with

   Julie Underwood

   Competing interests

   No competing interests declared

2. Julie Underwood

   Department of Dermatology, Duke University, Durham, United States

   Contribution

   Data curation, Investigation, Methodology, Writing—review and editing

   Contributed equally with

   Angel Morrow

   Competing interests

   No competing interests declared

3. Lindsey Seldin

   1. Department of Dermatology, Duke University, Durham, United States
   2. Department of Cell Biology, Duke University, Durham, United States

   Present address

   Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States

   Contribution

   Data curation, Formal analysis, Investigation, Methodology, Writing—review and editing

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0002-4995-1152

4. Taylor Hinnant

   1. Department of Dermatology, Duke University, Durham, United States
   2. Department of Cell Biology, Duke University, Durham, United States

   Contribution

   Data curation, Investigation, Methodology

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0002-8912-6851

5. Terry Lechler

   1. Department of Dermatology, Duke University, Durham, United States
   2. Department of Cell Biology, Duke University, Durham, United States

   Contribution

   Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Writing—original draft, Project administration, Writing—review and editing

   For correspondence

   terry.lechler@duke.edu

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0003-3901-7013

• 1,877

  views

• 287

  downloads

• 23

  citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.