TY - JOUR TI - PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD AU - Elguindy, Mahmoud M AU - Kopp, Florian AU - Goodarzi, Mohammad AU - Rehfeld, Frederick AU - Thomas, Anu AU - Chang, Tsung-Cheng AU - Mendell, Joshua T A2 - Ponting, Chris P A2 - Manley, James L A2 - Ulitsky, Igor A2 - Grote, Philip VL - 8 PY - 2019 DA - 2019/07/25 SP - e48625 C1 - eLife 2019;8:e48625 DO - 10.7554/eLife.48625 UR - https://doi.org/10.7554/eLife.48625 AB - NORAD is a conserved long noncoding RNA (lncRNA) that is required for genome stability in mammals. NORAD acts as a negative regulator of PUMILIO (PUM) proteins in the cytoplasm, and we previously showed that loss of NORAD or PUM hyperactivity results in genome instability and premature aging in mice (Kopp et al., 2019). Recently, however, it was reported that NORAD regulates genome stability through an interaction with the RNA binding protein RBMX in the nucleus. Here, we addressed the contributions of NORAD:PUM and NORAD:RBMX interactions to genome maintenance by this lncRNA in human cells. Extensive RNA FISH and fractionation experiments established that NORAD localizes predominantly to the cytoplasm with or without DNA damage. Moreover, genetic rescue experiments demonstrated that PUM binding is required for maintenance of genomic stability by NORAD whereas binding of RBMX is dispensable for this function. These data provide an important foundation for further mechanistic dissection of the NORAD-PUMILIO axis in genome maintenance. KW - NORAD KW - PUMILIO KW - RBMX KW - long noncoding RNA KW - genome stability KW - lncRNA JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -