TY - JOUR TI - NINscope, a versatile miniscope for multi-region circuit investigations AU - de Groot, Andres AU - van den Boom, Bastijn JG AU - van Genderen, Romano M AU - Coppens, Joris AU - van Veldhuijzen, John AU - Bos, Joop AU - Hoedemaker, Hugo AU - Negrello, Mario AU - Willuhn, Ingo AU - De Zeeuw, Chris I AU - Hoogland, Tycho M A2 - Carey, Megan R A2 - Wassum, Kate M A2 - Cai, Denise J VL - 9 PY - 2020 DA - 2020/01/14 SP - e49987 C1 - eLife 2020;9:e49987 DO - 10.7554/eLife.49987 UR - https://doi.org/10.7554/eLife.49987 AB - Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior. KW - miniscope KW - cerebellum KW - cerebral cortex KW - striatum KW - mouse KW - imaging JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -