TY - JOUR TI - Defining murine monocyte differentiation into colonic and ileal macrophages AU - Gross-Vered, Mor AU - Trzebanski, Sébastien AU - Shemer, Anat AU - Bernshtein, Biana AU - Curato, Caterina AU - Stelzer, Gil AU - Salame, Tomer-Meir AU - David, Eyal AU - Boura-Halfon, Sigalit AU - Chappell-Maor, Louise AU - Leshkowitz, Dena AU - Jung, Steffen A2 - MacPherson, Andrew J A2 - Rath, Satyajit A2 - Mueller, Christoph VL - 9 PY - 2020 DA - 2020/01/08 SP - e49998 C1 - eLife 2020;9:e49998 DO - 10.7554/eLife.49998 UR - https://doi.org/10.7554/eLife.49998 AB - Monocytes are circulating short-lived macrophage precursors that are recruited on demand from the blood to sites of inflammation and challenge. In steady state, classical monocytes give rise to vasculature-resident cells that patrol the luminal side of the endothelium. In addition, classical monocytes feed macrophage compartments of selected organs, including barrier tissues, such as the skin and intestine, as well as the heart. Monocyte differentiation under conditions of inflammation has been studied in considerable detail. In contrast, monocyte differentiation under non-inflammatory conditions remains less well understood. Here we took advantage of a combination of cell ablation and precursor engraftment to investigate the generation of gut macrophages from monocytes. Collectively, we identify factors associated with the gradual adaptation of monocytes to tissue residency. Moreover, comparison of monocyte differentiation into the colon and ileum-resident macrophages revealed the graduated acquisition of gut segment-specific gene expression signatures. KW - monocytes KW - macrophages KW - intestine KW - colon KW - ileum JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -