TY - JOUR TI - Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense AU - Krejčová, Gabriela AU - Danielová, Adéla AU - Nedbalová, Pavla AU - Kazek, Michalina AU - Strych, Lukáš AU - Chawla, Geetanjali AU - Tennessen, Jason M AU - Lieskovská, Jaroslava AU - Jindra, Marek AU - Doležal, Tomáš AU - Bajgar, Adam A2 - Banerjee, Utpal A2 - Banerjee, Utpal A2 - Theopold, Ulrich VL - 8 PY - 2019 DA - 2019/10/14 SP - e50414 C1 - eLife 2019;8:e50414 DO - 10.7554/eLife.50414 UR - https://doi.org/10.7554/eLife.50414 AB - Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly conserved, the metabolic remodeling of insect macrophages remains poorly understood. Here, we used adults of the fruit fly Drosophila melanogaster to investigate the metabolic changes that occur in macrophages during the acute and resolution phases of Streptococcus-induced sepsis. Our studies revealed that orthologs of Hypoxia inducible factor 1α (HIF1α) and Lactate dehydrogenase (LDH) are required for macrophage activation, their bactericidal function, and resistance to infection, thus documenting the conservation of this cellular response between insects and mammals. Further, we show that macrophages employing aerobic glycolysis induce changes in systemic metabolism that are necessary to meet the biosynthetic and energetic demands of their function and resistance to bacterial infection. KW - aerobic glycolysis KW - Warburg effect KW - polarization of macrophages KW - bacterial infection KW - HIF1α KW - immunometabolism JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -