TY - JOUR TI - Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii AU - Hunt, Alex AU - Russell, Matthew Robert Geoffrey AU - Wagener, Jeanette AU - Kent, Robyn AU - Carmeille, Romain AU - Peddie, Christopher J AU - Collinson, Lucy AU - Heaslip, Aoife AU - Ward, Gary E AU - Treeck, Moritz A2 - Akhmanova, Anna A2 - Frischknecht, Friedrich A2 - Meissner, Markus A2 - Insall, Robert A2 - Hu, Ke VL - 8 PY - 2019 DA - 2019/10/02 SP - e50598 C1 - eLife 2019;8:e50598 DO - 10.7554/eLife.50598 UR - https://doi.org/10.7554/eLife.50598 AB - Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell communication, daughter cell orientation and the juxtanuclear accumulation of actin, but only modest defects in synchronicity of division and no defect in the replication of the apicoplast. 3D electron microscopy reveals that loss of CAP results in a defect in formation of a normal central residual body, but parasites remain connected within the vacuole. This dissociates synchronicity of division and parasite rosetting and reveals that establishment and maintenance of the residual body may be more complex than previously thought. These results highlight the different spatial requirements for F-actin regulation in Toxoplasma which appear to be achieved by partially overlapping functions of actin regulators. KW - Toxoplasma gondii KW - parasitology KW - actin KW - cell biology KW - cyclase-associated protein JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -