TY - JOUR TI - Lamina-specific AMPA receptor dynamics following visual deprivation in vivo AU - Tan, Han L AU - Roth, Richard H AU - Graves, Austin R AU - Cudmore, Robert H AU - Huganir, Richard L A2 - Calabrese, Ronald L A2 - Van Hooser, Stephen D A2 - Wierenga, Corette J VL - 9 PY - 2020 DA - 2020/03/03 SP - e52420 C1 - eLife 2020;9:e52420 DO - 10.7554/eLife.52420 UR - https://doi.org/10.7554/eLife.52420 AB - Regulation of AMPA receptor (AMPAR) expression is central to synaptic plasticity and brain function, but how these changes occur in vivo remains elusive. Here, we developed a method to longitudinally monitor the expression of synaptic AMPARs across multiple cortical layers in awake mice using two-photon imaging. We observed that baseline AMPAR expression in individual spines is highly dynamic with more dynamics in primary visual cortex (V1) layer 2/3 (L2/3) neurons than V1 L5 neurons. Visual deprivation through binocular enucleation induces a synapse-specific and depth-dependent change of synaptic AMPARs in V1 L2/3 neurons, wherein deep synapses are potentiated more than superficial synapses. The increase is specific to L2/3 neurons and absent on apical dendrites of L5 neurons, and is dependent on expression of the AMPAR-binding protein GRIP1. Our study demonstrates that specific neuronal connections, across cortical layers and even within individual neurons, respond uniquely to changes in sensory experience. KW - AMPA receptor KW - in vivo imaging KW - primary visual cortex KW - cortical layer KW - visual deprivation KW - GRIP1 JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -