TY - JOUR TI - YAP regulates cell size and growth dynamics via non-cell autonomous mediators AU - Mugahid, Douaa AU - Kalocsay, Marian AU - Liu, Xili AU - Gruver, Jonathan Scott AU - Peshkin, Leonid AU - Kirschner, Marc W A2 - Pan, Duojia A2 - Bronner, Marianne E VL - 9 PY - 2020 DA - 2020/01/08 SP - e53404 C1 - eLife 2020;9:e53404 DO - 10.7554/eLife.53404 UR - https://doi.org/10.7554/eLife.53404 AB - The Hippo pathway regulates organ size, regeneration, and cell growth by controlling the stability of the transcription factor, YAP (Yorkie in Drosophila). When there is tissue damage, YAP is activated allowing the restoration of homeostatic tissue size. The exact signals by which YAP is activated are still not fully understood, but its activation is known to affect both cell size and cell number. Here we used cultured cells to examine the coordinated regulation of cell size and number under the control of YAP. Our experiments in isogenic HEK293 cells reveal that YAP can affect cell size and number by independent circuits. Some of these effects are cell autonomous, such as proliferation, while others are mediated by secreted signals. In particular CYR61, a known secreted YAP target, is a non-cell autonomous mediator of cell survival, while another unidentified secreted factor controls cell size. KW - YAP KW - Hippo KW - cell-cell communication KW - regeneration KW - non-cell autonomous JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -