TY - JOUR TI - A single-cell survey of Drosophila blood AU - Tattikota, Sudhir Gopal AU - Cho, Bumsik AU - Liu, Yifang AU - Hu, Yanhui AU - Barrera, Victor AU - Steinbaugh, Michael J AU - Yoon, Sang-Ho AU - Comjean, Aram AU - Li, Fangge AU - Dervis, Franz AU - Hung, Ruei-Jiun AU - Nam, Jin-Wu AU - Ho Sui, Shannan AU - Shim, Jiwon AU - Perrimon, Norbert A2 - LemaƮtre, Bruno A2 - Akhmanova, Anna A2 - LemaƮtre, Bruno VL - 9 PY - 2020 DA - 2020/05/12 SP - e54818 C1 - eLife 2020;9:e54818 DO - 10.7554/eLife.54818 UR - https://doi.org/10.7554/eLife.54818 AB - Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions. KW - blood KW - hemocytes KW - scRNA-seq KW - wounding KW - wasp infestation KW - immune response JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -