TY - JOUR TI - Tgfb3 collaborates with PP2A and notch signaling pathways to inhibit retina regeneration AU - Lee, Mi-Sun AU - Wan, Jin AU - Goldman, Daniel A2 - Gross, Jeffrey A2 - Cheah, Kathryn Song Eng A2 - Gross, Jeffrey VL - 9 PY - 2020 DA - 2020/05/12 SP - e55137 C1 - eLife 2020;9:e55137 DO - 10.7554/eLife.55137 UR - https://doi.org/10.7554/eLife.55137 AB - Neuronal degeneration in the zebrafish retina stimulates Müller glia (MG) to proliferate and generate multipotent progenitors for retinal repair. Controlling this proliferation is critical to successful regeneration. Previous studies reported that retinal injury stimulates pSmad3 signaling in injury-responsive MG. Contrary to these findings, we report pSmad3 expression is restricted to quiescent MG and suppressed in injury-responsive MG. Our data indicates that Tgfb3 is the ligand responsible for regulating pSmad3 expression. Remarkably, although overexpression of either Tgfb1b or Tgfb3 can stimulate pSmad3 expression in the injured retina, only Tgfb3 inhibits injury-dependent MG proliferation; suggesting the involvement of a non-canonical Tgfb signaling pathway. Furthermore, inhibition of Alk5, PP2A or Notch signaling rescues MG proliferation in Tgfb3 overexpressing zebrafish. Finally, we report that this Tgfb3 signaling pathway is active in zebrafish MG, but not those in mice, which may contribute to the different regenerative capabilities of MG from fish and mammals. KW - muller glia KW - stem cell KW - Tgfb KW - Smad KW - PP2A KW - notch JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -