TY - JOUR TI - TMAO, a seafood-derived molecule, produces diuresis and reduces mortality in heart failure rats AU - Gawrys-Kopczynska, Marta AU - Konop, Marek AU - Maksymiuk, Klaudia AU - Kraszewska, Katarzyna AU - Derzsi, Ladislav AU - Sozanski, Krzysztof AU - Holyst, Robert AU - Pilz, Marta AU - Samborowska, Emilia AU - Dobrowolski, Leszek AU - Jaworska, Kinga AU - Mogilnicka, Izabella AU - Ufnal, Marcin A2 - Mangoni, Arduino A A2 - Barton, Matthias A2 - Mangoni, Arduino A VL - 9 PY - 2020 DA - 2020/06/08 SP - e57028 C1 - eLife 2020;9:e57028 DO - 10.7554/eLife.57028 UR - https://doi.org/10.7554/eLife.57028 AB - Trimethylamine-oxide (TMAO) is present in seafood which is considered to be beneficial for health. Deep-water animals accumulate TMAO to protect proteins, such as lactate dehydrogenase (LDH), against hydrostatic pressure stress (HPS). We hypothesized that TMAO exerts beneficial effects on the circulatory system and protects cardiac LDH exposed to HPS produced by the contracting heart. Male, Sprague-Dawley and Spontaneously-Hypertensive-Heart-Failure (SHHF) rats were treated orally with either water (control) or TMAO. In vitro, LDH with or without TMAO was exposed to HPS and was evaluated using fluorescence correlation spectroscopy. TMAO-treated rats showed higher diuresis and natriuresis, lower arterial pressure and plasma NT-proBNP. Survival in SHHF-control was 66% vs 100% in SHHF-TMAO. In vitro, exposure of LDH to HPS with or without TMAO did not affect protein structure. In conclusion, TMAO reduced mortality in SHHF, which was associated with diuretic, natriuretic and hypotensive effects. HPS and TMAO did not affect LDH protein structure. KW - heart failure KW - trimethylamine N-oxide KW - seafood KW - gut bacteria KW - cardiovascular system JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -