Multiple Wnts act synergistically to induce Chk1/Grapes expression and mediate G2 arrest in Drosophila tracheoblasts
- Institute for Stem Cell Biology and Regenerative Medicine (inStem), India
- National Center for Biological Sciences, India
- Institute of Genetics and Molecular and Cellular Biology, France
- Institute for Stem Cell Science and Regenerative Medicine, India
Abstract
Larval tracheae of Drosophila harbor progenitors of the adult tracheal system (tracheoblasts). We showed previously that thoracic tracheoblasts arrest in the G2 phase of the cell cycle in an ATR-Checkpoint Kinase1(Chk1)-dependent manner prior to division and morphogenesis (Kizhedathu et al., 2018). Here we investigate developmental regulation of Chk1 activation. We report that Wnt signaling is high in tracheoblasts and is necessary for high levels of activated (phosphorylated) Chk1. We find that canonical Wnt signaling facilitates this by transcriptional upregulation of Chk1 in cells that have ATR kinase activity. Wnt signalling is dependent on four Wnts (Wg, Wnt5, 6,10) that are expressed at high levels in arrested tracheoblasts and downregulated at mitotic re-entry. Interestingly, none of the Wnts are dispensable and act synergistically to induce Chk1. Finally, we show that downregulation of Wnt signalling and Chk1 expression leads to mitotic re-entry and the concomitant upregulation of Dpp signalling, driving tracheoblast proliferation.
Data availability
All data generated or analysed during this study are included in the manuscript.
Article and author information
Author details
Funding
Department of Biotechnology , Ministry of Science and Technology (inStem Core Grant)
- Arjun Guha
Department of Biotechnology , Ministry of Science and Technology (InStem Core Grant)
- Arjun Guha
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Kizhedathu et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,460
- views
-
- 132
- downloads
-
- 7
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 7
- citations for umbrella DOI https://doi.org/10.7554/eLife.57056
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Multiple Wnts act synergistically to induce Chk1/Grapes expression and mediate G2 arrest in Drosophila tracheoblasts
eLife 9:e57056.
https://doi.org/10.7554/eLife.57056
