TY - JOUR TI - Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster AU - Yoshinari, Yuto AU - Ameku, Tomotsune AU - Kondo, Shu AU - Tanimoto, Hiromu AU - Kuraishi, Takayuki AU - Shimada-Niwa, Yuko AU - Niwa, Ryusuke A2 - Yamashita, Yukiko M A2 - Banerjee, Utpal A2 - Yamashita, Yukiko M A2 - Sun, Jianjun VL - 9 PY - 2020 DA - 2020/10/20 SP - e57101 C1 - eLife 2020;9:e57101 DO - 10.7554/eLife.57101 UR - https://doi.org/10.7554/eLife.57101 AB - Stem cells fuel the development and maintenance of tissues. Many studies have addressed how local signals from neighboring niche cells regulate stem cell identity and their proliferative potential. However, the regulation of stem cells by tissue-extrinsic signals in response to environmental cues remains poorly understood. Here we report that efferent octopaminergic neurons projecting to the ovary are essential for germline stem cell (GSC) increase in response to mating in female Drosophila. The neuronal activity of the octopaminergic neurons is required for mating-induced GSC increase as they relay the mating signal from sex peptide receptor-positive cholinergic neurons. Octopamine and its receptor Oamb are also required for mating-induced GSC increase via intracellular Ca2+ signaling. Moreover, we identified Matrix metalloproteinase-2 as a downstream component of the octopamine-Ca2+ signaling to induce GSC increase. Our study provides a mechanism describing how neuronal system couples stem cell behavior to environmental cues through stem cell niche signaling. KW - germline stem cell KW - stem cell niche KW - mating KW - octopamine KW - acetylcholine KW - matrix metalloproteinase JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -